A colloidal gold immunochromatography for the detection of flumioxazin residues in fruits. Issue 10 (14th September 2022)

• Record Type: Journal Article
• Authors: Wang, Peng; Xu, Xinxin; Liu, Liqiang; Song, Shanshan; Kuang, Hua; Xu, Chuanlai; Wu, Xiaoling
• Is Part Of: Journal of food science. Volume 87:Issue 10(2022)
• Page Start: 4538
• Page End: 4547
• Publication Date: 2022-09-14
• Subjects: flumioxazin -- fruit -- ICA strip -- qualitative detection -- quantitative detection; Food -- Periodicals; Food -- Research -- Periodicals; Food -- Periodicals; Research -- Periodicals; Levensmiddelen; Voeding; 664
• DOI: 10.1111/1750-3841.16288
• Languages: English
• ISSNs: 0022-1147

Abstract

Abstract: In this study, an excellent monoclonal antibody (mAb) was produced that could sensitively and specifically recognize Flu in fruit samples. The mAb belonged to the IgG2 subclass and had a half‐inhibitory concentration (IC50 ) of 0.59 ng/ml and a limit of detection (LOD) of 0.13 ng/ml. A cross‐reactivity test revealed that the mAb had good specificity for Flu. A gold nanoparticle‐based lateral‐flow immunochromatographic (ICA) strip was used for the rapid screening of Flu in fruit samples. The method had a visual cut‐off value of 10 µg/kg for Flu in three different fruit samples when evaluated with the naked eye. The LODs were 5.25, 3.13, and 4.64 µg/kg for Flu in apples, grapes, and oranges, respectively, as obtained with the aid of a strip scan reader. Therefore, this ICA strip assay represented a potential tool for the detection of Flu in fruit samples.