Prevalence of Virulence Genes in Extended‐Spectrum β‐lactamases (ESBLs)‐Producing Salmonella in Retail Raw Chicken in China. Issue 4 (25th March 2018)

• Record Type: Journal Article
• Authors: Qiao, Jing; Alali, Walid Q.; Liu, Jiangshan; Wang, Yaping; Chen, Sheng; Cui, Shenghui; Yang, Baowei
• Is Part Of: Journal of food science. Volume 83:Issue 4(2018)
• Page Start: 1048
• Page End: 1052
• Publication Date: 2018-03-25
• Subjects: extended‐spectrum β‐lactamase (ESBL) -- retail chicken -- Salmonella -- virulence genes; Food -- Periodicals; Food -- Research -- Periodicals; Food -- Periodicals; Research -- Periodicals; Levensmiddelen; Voeding; 664
• DOI: 10.1111/1750-3841.14111
• Languages: English
• ISSNs: 0022-1147

Abstract

Abstract: Extended‐spectrum β‐lactamases (ESBLs)‐producing Salmonella is a tremendous hazard to food safety and public health. The objective of this study was to determine the prevalence of 30 virulence genes ( avr A, sip A, sse C, mar T, rhu M, sii E, pip A, pip D, env R, gog B, gtg A, sod C1, sse I, irs A, sop E2, spv C, rck, spv R, fhu A, msg A, pag K, srfj, stkc, fim A, lpf D, pef A, stc C, ste B, stj B, and tcf A) in 156 ESBLs‐producing Salmonella isolates that belonged to 21 serotypes. These isolates were recovered from retail raw chicken samples collected from 5 provinces and 2 national cities in China between 2007 and 2012. The results indicated that 154 (98.7%) ESBLs‐producing Salmonella isolates carried at least 1 virulence gene, 138 (88.5%) simultaneously carried at least 5 virulence genes, 107 (68.6%) carried 10 or more, and 20 (12.8%) carried 15 or more virulence genes. The most frequently detected virulence genes were mar T ( n = 127, 81.4%), sii E ( n = 126, 80.8%), msg A ( n = 121, 77.6%), and sip A ( n = 121, 77.6%). Significant difference was identified between detection percentages of virulence genes of rhu M, pip D, env R, sop E2, pag K, lpf D, ste B, and stj B in S . Indiana, S . Thompson, S . Enteritidis, S . Typhimurium, S . Shubra, S . Edinburg, and S . Agona isolates. Distribution of virulence genes were significantly influenced by sampling districts ( P < 0.01), especially for sod C1 and pip D, and then were msg A and sop E2. The heatmap showed the frequencies of virulence genes in ESBLs‐producing isolates from retail chickens in southern, central, and northern regions of China were completely different from each other. Based on our findings, ESBLs‐producing Salmonella of retail chicken origin were common carriers of multiple virulence genes and were regionally distributed.