Investigation of pectolytic and PR genes expression, quality and phytochemical contents in organic and non-organic table grapes at harvest and during storage. (May 2023)

• Record Type: Journal Article
• Authors: Song, Huwei; Asghari, Mohammadreza; Zahedipour-Sheshglani, Pari; Diao, Enjie; Xiang, Xinran; Liang, Xiaona; Abdollahi Mandoulakani, Babak; Qian, Shiquan
• Is Part Of: Food research international. Volume 167(2023)
• Publication Date: 2023-05
• Subjects: Organic -- Tyrosine ammonia lyase -- Total proteins -- VvPME -- VvPG -- VvCHI -- VvGNS; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2023.112717
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Changes in pectolytic and PR-genes expression, quality and phytochemical contents of organic and conventional table grapes Highlights: Lytic and PR genes expression was rapidly increased in organic grapes during storage. Organic grapes showed higher phenolics and flavonoids at harvest and during storage. Decay extension, MDA, EL contents and firmness showed no difference. Higher antioxidants and H2 O2 content was recorded in organic samples. Pectolytic genes are involved in resistance mechanisms of organic grapes. Abstract: The demand for organic table grapes is increasing worldwide. However, comprehensive information of quality parameters and phytochemical compounds in organically grown fruit remain unclear. Furthermore, table grapes are perishable and postharvest quality retention and waste prevention is very important. In this study we have compared the differences between organic and non-organic table grapes in terms of phytochemical compounds and quality parameters as well as the changes in the expression levels of pathogen related and lytic genes during storage. Organic fruit showed higher levels of phenolics, flavonoids, caffeic acid, hydrogen peroxide, protein content, antioxidant and anti-stress enzymes and total antioxidant activities at harvest and during storage. Although, the expression levels of polygalactronases, pectin methyl esterase, chitinase and glucanase genes was lower in organically grown table grapes at harvest, but the expression of all these genes was significantly increased during cold storage. After 60 days of cold storage the expression levels of pectin methyl esterase, chitinase and glucanase genes was significantly higher than the conventionally grown grape berries in organic ones. The highest expression of polygalacturonase was recorded in organic samples after 30 days of storage. There was no significant difference between the two types of table grapes for decay extension and tissue deterioration rate. The results of this study indicate that due to higher levels of phytochemicals and antioxidant compounds the organic table grapes have a higher nutritional quality. Furthermore, the increase in PR and pectolytic genes expression levels is enough for decreasing the fruit susceptibility to decay pathogens and enhancing the postharvest life of organic grapes.