Inulin reduces liver triacylglycerol by increasing lipid droplet lipolysis in fat-loaded mice. (January 2023)

• Record Type: Journal Article
• Authors: Chen, Bingbing; Shi, Yumeng; Zhang, Kai; Chang, Yanzhong; Fu, Pengcheng; Liu, Pingsheng; Zhang, Shuyan
• Is Part Of: Food research international. Volume 163(2023)
• Publication Date: 2023-01
• Subjects: Lipid droplet -- Metabolic disorders -- Inulin -- High-fat-diet -- Gut microbiota -- Fat loaded short- and long-term; LD lipid droplet -- ATGL adipose triglyceride lipase -- HFD high-fat diet -- ITF inulin-type fructan -- DP degree of polymerization -- VCO2 CO2 production -- VO2 O2 consumption -- RER respiratory exchange rate -- HE heat expenditure -- CHO cholesterol -- TAG triacylglycerol -- LDL-C low-density lipoprotein cholesterol -- HDL-C high-density lipoprotein cholesterol -- ALT alanine transaminase -- OGTT oral glucose tolerance test -- BAT brown adipose tissue -- WAT white adipose tissue -- PMSF phenylmethanesulfonyl fluoride -- OTU operational taxonomic unit -- PCoA principal coordinates analysis; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.112226
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Inulin supplementation could alleviate high-fat diet-induced hepatic steatosis, mediated through increasing ATGL on liver lipid droplets. The beneficial effects of inulin, such as increasing energy expenditure, alleviating adiposity, and improving glucose intolerance, could only be achieved in mice fed with high-fat diet for 1 month, not for 4 months. Gut microbiota in the short- and long-term fat-loaded mice was shown to be modulated in a different way, which may mediate the differential effects of inulin. Abstract: Increased consumption of high-fat low-fiber foods has been shown to contribute to the development of metabolic syndromes, such as fatty liver, obesity, diabetes, et al . Fermentable dietary fiber, such as inulin, is broadly used to mitigate host metabolic abnormalities. In this work, we studied systematically the effect of inulin on mice with metabolic disorders, induced by either short- or long-term high-fat feeding. As expected, inulin reduced the body weight of mice in both groups. However, it was found that inulin feeding could only increase energy expenditure, alleviate adiposity, and improve glucose intolerance in mice fed with high-fat diet (HFD) for 1 month but not for 4 months. Surprisingly, inulin supplementation could alleviate HFD-induced hepatic steatosis, mediated through increasing adipose triglyceride lipase (ATGL) on liver lipid droplets, in both groups. Gut microbiota in the short- and long-term fat-loaded mice were shown to be modulated differently, which may mediate the differential effects of inulin. These results may help in understanding the role and mechanism of fermentable fiber regulating host metabolism.