Α-Dicarbonyl compounds trapping ability and antiglycative effect of high-molecular-weight brewer's spent grain melanoidins. (15th April 2023)

• Record Type: Journal Article
• Authors: Blidi, Slim; Troise, Antonio Dario; Ledbetter, Moira; Cottin, Sarah; Sturrock, Keith; De Pascale, Sabrina; Scaloni, Andrea; Fiore, Alberto
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 180(2023)
• Publication Date: 2023-04-15
• Subjects: Melanoidins -- α-dicarbonyl compounds -- Brewer's spent grain -- Polyphenols -- Advanced glycation end-products; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2023.114679
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Polyphenols participate in the Maillard reaction pathways scavenging α-dicarbonyl compounds (DCs) and contributing to the mitigation of carbonyl burden through dietary exposure/routes. The current study demonstrated the effectiveness of high-molecular-weight brewer's spent grain melanoidins (HMW-BSGM) in reacting with DCs in an in vitro model system. HMW-BSGM (4 mg/mL) quenched more than 95% of glyoxal and methylglyoxal, and more than 80% of 2, 3-butanedione after a 7-day incubation at 37 °C. Among tested polyphenols, sinapic acid showed the highest trapping capacity with inhibition rates of 33.1, 49.1 and 49.3% for glyoxal, methylglyoxal and 2, 3-butanedione because of hydroxyalkylation reaction as revealed by liquid chromatography high-resolution tandem mass spectrometry experiments. The formation of free fluorescent AGEs was substantially hindered (79.3%) by HMW-BSGM (4 mg/mL). These findings corroborate the hypothesis that the accumulation of polyphenols in melanoidins skeleton can hinder undesired effects and potentially harmful reactions involving α-dicarbonyl compounds. Graphical abstract: Image 1 Highlights: A novel use of brewer's spent gain is discussed. The polyphenolic profile of brewer's spent grain melanoidins is established. Brewer's spent grain melanoidins trapped α-dicarbonyls in a simplified model system. Brewer's spent grain melanoidins inhibited the formation of free fluorescent AGEs. The putative adduct of sinapic acid-di-MGO is reported for the first time.