Α-Dicarbonyl compounds trapping ability and antiglycative effect of high-molecular-weight brewer's spent grain melanoidins. (15th April 2023) - Blidi, Slim
Abstract: Polyphenols participate in the Maillard reaction pathways scavenging α-dicarbonyl compounds (DCs) and contributing to the mitigation of carbonyl burden through dietary exposure/routes. The current study demonstrated the effectiveness of high-molecular-weight brewer's spent grain melanoidins (HMW-BSGM) in reacting with DCs in an in vitro model system. HMW-BSGM (4 mg/mL) quenched more than 95% of glyoxal and methylglyoxal, and more than 80% of 2, 3-butanedione after a 7-day incubation at 37 °C. Among tested polyphenols, sinapic acid showed the highest trapping capacity with inhibition rates of 33.1, 49.1 and 49.3% for glyoxal, methylglyoxal and 2, 3-butanedione because of hydroxyalkylation reaction as revealed by liquid chromatography high-resolution tandem mass spectrometry experiments. The formation of free fluorescent AGEs was substantially hindered (79.3%) by HMW-BSGM (4 mg/mL). These findings corroborate the hypothesis that the accumulation of polyphenols in melanoidins skeleton can hinder undesired effects and potentially harmful reactions involving α-dicarbonyl compounds. Graphical abstract: Image 1 Highlights: A novel use of brewer's spent gain is discussed. The polyphenolic profile of brewer's spent grain melanoidins is established. Brewer's spent grain melanoidins trapped α-dicarbonyls in a simplified model system. Brewer's spent grain melanoidins inhibited the formation of free fluorescent AGEs. The putative adduct of sinapic acid-di-MGO is reported for the first time.