Integrative analyses of transcriptome and metabolome reveal comprehensive mechanisms of Epigallocatechin-3-gallate (EGCG) biosynthesis in response to ecological factors in tea plant (Camellia sinensis). (April 2023)

• Record Type: Journal Article
• Authors: Xiang, Ping; Zhu, Qiufang; zhang, Luhuan; Xu, Puzhen; Liu, Lijia; Li, Yuanyuan; Cheng, Bosi; Wang, Xingjian; Liu, Jianghong; Shi, Yutao; Wu, Liangyu; Lin, Jinke
• Is Part Of: Food research international. Volume 166(2023)
• Publication Date: 2023-04
• Subjects: Tea -- Camellia sinensis -- Epigallocatechin-3-gallate -- EGCG -- Flavonoid -- Phenolic acid -- Biosynthesis -- Ecological factor -- Regulation -- Temperature -- Light intensity -- Humidity; 4CL 4-coumaroyl-CoA ligase -- ANR anthocyanidin reductase -- ANS anthocyanidin synthase -- C4H cinnamate 4-hydroxylase -- CHI chalcone isomerase -- D4P d-erythrose-4-phosphate -- DAMs differentially accumulated metabolites -- DEGs differentially expressed genes -- DFR dihydroflavonol 4-reductase -- DHQ/SDH 3-dehydroquinate dehydratase/shikimate 5-dehydrogenase -- EGC epigallocatechin -- EGCG epigallocatechin-3-gallate -- F3′5′H flavonoid 3′, 5′-hydroxylase -- F3′H flavonoid 3′-hydroxylase -- F3H flavanone 3-hydroxylase -- FPKM Fragments Per Kilobase of exon model in the Million mapped fragments -- GA gallic acid -- GO Gene Ontology -- KEGG Kyoto Encyclopedia of Genes and Genomes -- LAR leucoanthocyanidin reductase -- PAL phenylalanine ammonia-lyase -- PCA principal component analysis -- PEP phosphoenolpyruvic acid -- PHE phenylalanine -- RH relative humidity -- SA shikimic acid -- SCPL serine carboxypeptidase-like -- TFs transcription factors -- UPLC/MS-MS ultraperformance liquid chromatography-tandem mass spectrometry -- WGCNA weighted gene co-expression network analyses; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2023.112591
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: The metabolic flux was converted from phenolic acid to flavonoid biosynthesis pathway under the appropriately environmental conditions. EGCG biosynthesis in tea plant was comprehensively regulated in response to ecological factors by a series of five structural genes, 11 miRNAs, and seven transcription factors. Temperature plays a prominent role in EGCG biosynthesis in response to ecological factors, and the optimal environmental conditions obtained for EGCG biosynthesis were 28℃, 70 % relative humidity of the substrate, and 280 µmol·m −2 ·s −1 light intensity. Abstract: Epigallocatechin-3-gallate (EGCG), a flavoured and healthy compounds in tea, is affected by the ecological factors. However, the biosynthetic mechanisms of EGCG in response to the ecological factors remian unclear. In this study, a response surface method with a Box-Behnken design was used to investigate the relationship between EGCG accumulation and ecological factors; further, integrative transcriptome and metabolome analyses were performed to explore the mechanism underlying EGCG biosynthesis in response to environmental factors. The optimal environmental conditions obtained for EGCG biosynthesis were as follows: 28℃, 70 % relative humidity of the substrate, and 280 µmol·m −2 ·s −1 light intensity; the EGCG content was increased by 86.83 % compared to the control (CK1 ). Meanwhile, the order of EGCG content in response to the interaction of ecological factors was as follows: interaction of temperature and light intensity > interaction of temperature and relative humidity of the substrate > interaction of light intensity and relative humidity of the substrate, indicating that temperature was the dominant ecological factors. EGCG biosynthesis in tea plants was found to be comprehensively regulated by a series of structural genes ( CsANS, CsF3H, CsCHI, CsCHS, and CsaroDE ), miRNAs (miR164, miR396d, miR5264, miR166a, miR171d, miR529, miR396a, miR169, miR7814, miR3444b, and miR5240), and transcription factors ( MYB93, NAC2, NAC6, NAC43, WRK24, bHLH30, and WRK70 ); further, the metabolic flux was regulated and converted from phenolic acid to the flavonoid biosynthesis pathway based on accelerated consumption of phosphoenolpyruvic acid, d -erythrose-4-phosphate, and l -phenylalanine in response to ambient changes in temperature and light intensity. Overall, the results of this study reveal the effect of ecological factors on EGCG biosynthesis in tea plants, providing novel insights for improving tea quality.