Genome-wide transcriptome analysis uncovers gene networks regulating fruit quality and volatile compounds in mango cultivar 'Tainong' during postharvest. (March 2023)

• Record Type: Journal Article
• Authors: Li, Li; Yi, Ping; Sun, Jian; Tang, Jie; Liu, Guoming; Bi, Jinfeng; Teng, Jianwen; Hu, Meijiao; Yuan, Fang; He, Xuemei; Sheng, Jinfeng; Xin, Ming; Li, Zhichun; Li, Changbao; Tang, Yayuan; Ling, Dongning
• Is Part Of: Food research international. Volume 165(2023)
• Publication Date: 2023-03
• Subjects: Mango -- Transcriptome -- Fruit ripening -- Flavor -- Volatile compound -- Regulatory network; HS-GC-IMS headspace gas chromatography and ion mobility spectrometry -- WGCNA Weighted gene co-expression network analysis -- SNARE soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors -- STEM Short Time Series Expression Miner -- MDA malonaldehyde -- SOD superoxide dismutase -- POD peroxidase -- CAT catalase -- PE pectinesterase -- TSS total soluble solids -- TA titratable acid -- RI retention index -- NGS next-generation sequencing -- RPKM reads per kilobase per million mapped reads -- PCA Principal component analysis -- FDR false discovery rate -- DEGs differentially expressed genes -- GO Gene Ontology -- KEGG Kyoto Encyclopedia of Genes and Genomes -- qRT-PCR Quantitative Real-Time PCR -- TCA tricarboxylic acid cycle -- MVA mevalonic acid -- ROS reactive oxygen species -- GAPs GPI-anchored proteins -- GPI glycosylphosphatidylinositol; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2023.112531
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Terpenoids were significantly altered during mango fruit ripening. Cysteine and methionine metabolism were activated during mango ripening. Gene regulatory network from pulp to peel in mango ripening was constructed. Abstract: Mango is one of the most economically important fruit; however, the gene regulatory mechanism associated with ripening and quality changes during storage remains largely unclear. This study explored the relationship between transcriptome changes and postharvest mango quality. Fruit quality patterns and volatile components were obtained using headspace gas chromatography and ion-mobility spectrometry (HS-GC-IMS). The changes in mango peel and pulp transcriptome were analyzed during four stages (pre-harvesting, harvesting, maturity, and overripe stages). Based on the temporal analysis, multiple genes involved in the biosynthesis of secondary metabolites were upregulated in both the peel and pulp during the mango ripening process. Moreover, cysteine and methionine metabolism related to ethylene synthesis were upregulated in the pulp over time. Weighted gene co-expression network analysis (WGCNA) further showed that the pathways of pyruvate metabolism, citrate cycle, propionate metabolism, autophagy, and SNARE interactions in vesicular transport were positively correlated with the ripening process. Finally, a regulatory network of important pathways from pulp to peel was constructed during the postharvest storage of mango fruit. The above findings provide a global insight into the molecular regulation mechanisms of postharvest mango quality and flavor changes.