Nanoemulsion: A novel delivery approach for thermosensitive IgG on inhibiting milk fat oxidation. (March 2023)

• Record Type: Journal Article
• Authors: Zhang, An-Qi; Liu, Meng-Qi; Li, Xiao-Yan; Xu, Di; Yin, Yu-Qi; Song, Nan-Nan; Zhang, Ying-Hua
• Is Part Of: Food research international. Volume 165(2023)
• Publication Date: 2023-03
• Subjects: Nanoemulsion -- Immunoglobulin G -- Heat resistance -- Acid resistance; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2023.112545
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Provide a new method to protect heat-sensitive immunoglobulin G. Nanoemulsion can effectively avoid immunoglobulin G being destroyed by stomach acid. Immunoglobulin G can effectively inhibit the peroxidation of fat in milk. Abstract: IgG, a biologically active substance in bovine colostrum, is easily inactivated during heat treatment and edible process to lose its biological activity. Nanoemulsion can effectively protect IgG to maintain its biological activity from injurious treatment. In this study, a food-grade nanoemulsion system was developed to protect IgG from heat and acid damage. It can be found that the residual rate of nanoemulsion-protected IgG reaches 87.1 % after 10 min at 72 °C. After 5 min at 82 °C, the residual rate of IgG in nanoemulsion was 18.7 % higher than that in PBS. In the simulated gastric fluid at pH 2.0, the residual rate of IgG in the nanoemulsion reacted for 4 h was 21.5 % higher than that in PBS. It indicated that nanoemulsion system can improve the heat and acid resistance of IgG compared with others, which is attributed to the lowest water activity of nanoemulsion. The contents of hydroperoxide and malondialdehyde in the milk after storage for 72 h with nanoemulsion-protected IgG were 0.12 meq/kg and 0.04 mg/kg, respectively, less than that of PBS-protected IgG. IgG is protected by nanoemulsion can effectively protect its activity during processing, which provides a theoretical basis for its direct application in liquid milk.