LC-IMS-HRMS for identification of biomarkers in untargeted metabolomics: The effects of pterostilbene and resveratrol consumption in liver steatosis, animal model. (March 2023)

• Record Type: Journal Article
• Authors: Lacalle-Bergeron, Leticia; Izquierdo-Sandoval, David; Fernández-Quintela, Alfredo; Portillo, María P.; Sancho, Juan Vicente; Hernández, Félix; Portolés, Tania
• Is Part Of: Food research international. Volume 165(2023)
• Publication Date: 2023-03
• Subjects: Untargeted metabolomics -- Ion mobility -- HRMS -- Resveratrol -- Pterostilbene -- Liver steatosis; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.112376
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Untargeted metabolomics-based IMS-HRMS is a powerful approach for unravel biomarkers identification. Pterostilbene and resveratrol diet supplementation revealed significant changes in liver metabolism. 34 compounds related to pterostilbene and resveratrol diet supplementation were tentatively identified. Aligned HDMS E spectra, CCS databases and CCS predictions tools helped in the structural characterisation. Abstract: Untargeted metabolomics with the combination of ion mobility separation coupled to high resolution mass spectrometry (IMS-HRMS) was applied to investigate the impact of resveratrol and pterostilbene supplementation on the metabolic fingerprint of the Wistar rats liver with induced liver steatosis. RP-LC and HILIC in both ionisation modes were employed to analyse the liver samples (n = 40) from Wistar rats fed with a high-fat and high-fructose diet, supplemented or not with resveratrol and pterostilbene. After univariate and multivariate statistical analysis, 34 metabolites were highlighted in the different diets and elucidated. Despite the structural similarity, different alterations in liver metabolism were observed by the supplementations. Resveratrol treatment was characterised by the alteration in metabolism of 17 lysophospholipids, while pterostilbene affected some vitamins and derivatives, among others. IMS has demonstrated great potential in the elucidation process thanks to the additional structural descriptor the CCS (Å 2 ), providing more confidence in the identification.