The intervention effects of konjac glucomannan with different molecular weights on high-fat and high-fructose diet-fed obese mice based on the regulation of gut microbiota. (March 2023)

• Record Type: Journal Article
• Authors: Liu, Qian; Fang, Jie; Huang, Wenqi; Liu, Sining; Zhang, Xueting; Gong, Guiping; Huang, Linjuan; Lin, Xiaoliang; Wang, Zhongfu
• Is Part Of: Food research international. Volume 165(2023)
• Publication Date: 2023-03
• Subjects: Konjac glucomannan -- Structure–activity relationships -- High–calorie diet -- Obesity -- Gut microbiota; HFFD high-fat and high-fructose diet -- KGM Konjac Glucomannan -- SCFAs short-chain fatty acids -- GTT glucose tolerance test -- HOMA-IR homeostasis model assessment of insulin resistance -- TG triglyceride -- TC total cholesterol -- LDL-C low density lipoprotein cholesterol -- HE hematoxylin and eosin -- PPARγ peroxisome proliferator activated receptor gamma -- LPL lipoprotein lipase -- Fas fatty acid synthase -- Cpt1 carnitine palmitoyl transferase 1 -- Srebp sterol regulatory element-binding protein -- HSL hormone sensitive lipase -- OTUs operational taxonomic units; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2023.112498
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Compared with 5 kDa KGM-2 and 1 kDa KGM-3, dietary supplement with 90 kDa KGM-1 exerted stronger weight loss activity. KGM-1 down-regulated Pparg expressions, and promoted Hsl and Cpt-1 expressions, thereby reducing liver lipids accumulation. The more potential weight loss effect of KGM-1 maybe attributed to its regulation of gut microbiota. Abstract: Konjac is a high-quality dietary fiber rich in β-glucomannan, which has been reported to possess anti-obesity effects. To explore the effective components and the structure–activity relationships of konjac glucomannan (KGM), three different molecular weight components (KGM-1 (90 kDa), KGM-2 (5 kDa), KGM-3 (1 kDa)) were obtained, and systematical comparisons of their effects on high-fat and high-fructose diet (HFFD)-induced obese mice were investigated in the present study. Our results indicated that KGM-1, with its larger molecular weight, reduced mouse body weight and improved their insulin resistance status. KGM-1 markedly inhibited lipid accumulation in mouse livers induced by HFFD by downregulating Pparg expression and upregulating Hsl and Cpt1 expressions. Further investigation revealed that dietary supplementation with konjac glucomannan at different molecular weights caused β-diversity changes in gut microbes. The potential weight loss effect of KGM-1 maybe attributed to the abundance of changes in Coprobacter, Streptococcus, Clostridium IV, and Parasutterella. The results provide a scientific basis for the in-depth development and utilization of konjac resources.