Polysaccharides from Atractylodes macrocephala Koidz. Ameliorate ulcerative colitis via extensive modification of gut microbiota and host metabolism. (December 2020)

• Record Type: Journal Article
• Authors: Feng, Wuwen; Liu, Juan; Tan, Yuzhu; Ao, Hui; Wang, Jing; Peng, Cheng
• Is Part Of: Food research international. Volume 138(2020)Part B
• Publication Date: 2020-12
• Subjects: Atractylodes macrocephala Koidz. -- Polysaccharides -- Ulcerative colitis -- Gut microbiota -- Metabolomics; AM Atractylodes macrocephala Koidz. -- AMP Polysaccharides from Atractylodes macrocephala Koidz. -- DSS dextran sulfate sodium -- HPLC high performance liquid chromatography -- KEGG Kyoto Encyclopedia of Genes and Genomes -- LEfSe linear discriminate analysis effect size -- NMDS nonmetric multi-dimensional scaling -- OUT operational taxonomic units -- PCA Principal component analysis -- PICRUSt phylogenetic investigation of communities by reconstruction of unobserved states -- PLS-DA partial least squares discrimination analysis -- SCFAs short-chain fatty acids -- UC ulcerative colitis -- UPLC-MS/MS ultra-high-performance liquid chromatography coupled with mass spectrometry/mass spectrometry -- VIP variable importance in the projection; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2020.109777
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Polysaccharides from Atractylodes macrocephala Koidz. (AMP) dose dependently ameliorate UC induced by DSS. AMP can reverse the perturbed composition of gut microbiota. AMP can restore the disturbed fecal and plasma metabolites, including SCFAs, amino acids, bile acids, cadaverine, etc. An integrative microbiome-metabolomics network was constructed. Abstract: Turbulence of gut microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids is an important factor in the development of diseases. Many polysaccharides are effective on diseases including ulcerative colitis (UC), yet most studies investigating the mechanisms of polysaccharides mainly focused on their effects on gut microbiota composition and SCFAs, and other metabolites of gut microbiota are often neglected. Here, we examined the effects of polysaccharides from Atractylodes macrocephala Koidz. (AMP) on experimental UC induced by dextran sulfate sodium (DSS) and explored underlying mechanisms of AMP by 16S rDNA-based gut microbiota analysis and untargeted fecal and plasma metabolomics. In addition, a multiscale, multifactorial network was constructed to visualize the mechanisms of AMP. The results showed that AMP significantly increased body weight and ameliorated colonic injury in DSS treated mice. AMP also partly restored the perturbed gut microbiota composition induced by DSS. Untargeted fecal and plasma metabolomics showed that AMP can not only modulate the production of SCFAs by gut microbiota, but also the ability to digest food nutrients, metabolism of amino acids and bile acids, production of cadaverine and other metabolites by hosts and gut microbiota. The study demonstrated that, in addition to SCFAs, AMP can extensively modulate the metabolism of gut microbiota and hosts to achieve the therapeutic effects. This study adds new mechanisms of polysaccharides in treating diseases.