Colloidal transport of lipid digesta in human and porcine small intestinal mucus. (December 2020)

• Record Type: Journal Article
• Authors: Macierzanka, Adam; Ménard, Olivia; Dupont, Didier; Gutkowski, Krzysztof; Staroń, Robert; Krupa, Lukasz
• Is Part Of: Food research international. Volume 138(2020)Part A
• Publication Date: 2020-12
• Subjects: Nutrient transport -- Dynamic digestion model -- Mucus barrier -- Lipolysis -- Proteolysis -- Particle diffusion; CLSM confocal laser scanning microscopy -- D diffusion coefficient -- DG diglyceride -- FFA free fatty acid -- GI gastrointestinal -- MG monoglyceride -- TG triglyceride; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2020.109752
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Small intestinal mucus showed a sieve-like behaviour when exposed to lipid digesta. Digesta penetration profiles of mucus are similar for adult human and adult pig. Adult pig mucus is a human-relevant substitute for mucus transport studies. Neonatal, piglet mucus is a less effective barrier to passively diffusing digesta. Abstract: Small intestinal mucus transport of food-derived particulates has not been extensively studied, despite mucus being a barrier nutrients need to cross before absorption. We used complex dispersions of digesta obtained from simulated, dynamic gastrointestinal digestion of yogurt to examine the penetrability of human and porcine mucus to the particles formed of lipolysis products. Quantitative, time-lapse confocal microscopy revealed a sieve-like behaviour of the pig jejunal and ileal mucus. The digesta diffusivity decreased significantly over the first 30 min of mucus penetration, and then remained constant at ca. 5 × 10 -12 m 2 s −1 (approx. 70% decrease from initial values). A non-significantly different penetrability was recorded for the ileal mucus of adult humans. The digesta diffusion rates in neonatal, jejunal mucus of 2 week old piglets were 5–8 times higher than in the three different types of adult mucus. This is the first report that validates the mucus of fully-grown pigs as a human-relevant substitute for mucus permeation studies of nutrients/bio-actives and/or complex colloidal dispersions (e.g., post-digestion food particulates, orally-administrated delivery systems).