Integrating transcriptomic and metabolomic analysis to understand muscle qualities of red swamp crayfish (Procambarus clarkii) under transport stress. (February 2023)

• Record Type: Journal Article
• Authors: Shi, Gangpeng; Gao, Tianqi; Li, Xuehong; Shi, Liu; Chen, Sheng; Ding, Anzi; Li, Xin; Qiao, Yu; Liao, Li; Xiong, Guangquan; Wang, Lan; Wu, Wenjin
• Is Part Of: Food research international. Volume 164(2023)
• Publication Date: 2023-02
• Subjects: Transport stress -- Meat qualities -- Red swamp crayfish (Procambarus clarkii) -- Omics; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.112361
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: The survival of crayfish after crowding stress was associated with the ribosomal protein pathway. Crowding stress resulted in a decline in water holding capacity of crayfish tail meat. Comparing to crayfish tail meat, hepatopancreas is susceptible to oxidative stress. Crowding stress leaded to the disorder of cell membrane and mitochondrial function of crayfish. These findings might help in understanding the molecular mechanisms of crayfish flesh under crowding stress in crayfish. Abstract: This study investigated the transport stress (crowding stress and duration) on the physicochemical properties, energy metabolism and antioxidant enzyme activities of the red swamp crayfish ( Procambarus clarkii ) tail muscle (CTM). Besides, transcriptomic and metabolomic were conducted to elucidate the possible mechanism of CTM alternations during transport stress. The survival rate of crayfish gradually decreased with the external crowding stress and crowding time increasing. The transport stress also led to the increased distance among muscle fibers, water mobility and energy consumption, and the decreased of water holding capacity (WHC), hardness of CTM. The hepatopancreas exhibited more sensitive to crowding stress than muscle. The multi-omics analysis revealed that transport stress could interfere the translation and protein folding functions of ribosomal proteins, fatty acid metabolism and degradation, physiological functions of mitochondria in CTM. This study could provide critical information to increase the understanding of the regulation mechanism of crayfish when subjected to transport stress.