1H NMR-based metabolic profile and chemometric analysis for the discrimination of Passiflora species genotypic variations. (February 2023)

• Record Type: Journal Article
• Authors: Dutra, Livia Macedo; Henrique Vieira Teles, Pedro; Diego da Conceição Santos, Alan; Franklin de Melo, Natoniel; Nagata, Noemi; Roberto Guedes da Silva Almeida, Jackson
• Is Part Of: Food research international. Volume 164(2023)
• Publication Date: 2023-02
• Subjects: Passiflora -- 1H NMR -- Chemometrics -- Metabolic profile -- Biological activities -- Quality control; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.112441
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Principal components analysis allowed discrimination of Passiflora extracts. Flavonoids played an important role in the grouping of Passiflora species. P. setacea and P. cincinnata had chemical composition similar to their varieties. Only P. alata was differentiated from its genetic variety. 1 H NMR can help in studies of novel alternatives for official Passiflora herbs. Abstract: The species of the genus Passiflora (Passifloraceae family) have been used as food, cosmetic and traditional herbal. As a result, the Passiflora species are widely cultivated and has an economic, medicinal and ornamental importance. The popular designation as "passion fruit" and chemical profile of several Passiflora species remains unknown. The lack of chemical information contributes to the erroneous classification and adulteration. In recent years, special attention has been paid to the bioactivity and phytochemical profiles of several Passiflora species extracts. In this research, 1 H NMR-based metabolic profiling coupled with chemometric tools was used to characterize and distinguish extracts obtained from different wild Passiflora species ( P. alata, P. cincinnata, and P. setacea ) and genetic varieties ( P. alata var. BRS Pérola do Cerrado, P. cincinnata var. BRS Sertão Forte, and P. setacea var. BRS Pérola do Cerrado). Fourteen metabolites were identified by 1D and 2D NMR experiments, highlighting the presence of fatty acids, carbohydrates, saponins, alkaloids, and mainly C-glycosidic flavones. Principal components analysis (PCA) allowed discrimination of Passiflora extracts, which the quadranguloside, oleanolic acid-3-sophoroside, α -glucose, β -glucose, and vitexin-2- O "-rhamnoside were relevant in the differentiation of P. alata and P. alata var. BRS Pérola do Cerrado, while the flavones isovitexin and isovitexin-2- O "-xyloside were dominant in the grouping of P. setacea and P. setacea var. BRS Pérola do Cerrado, and finally P. cincinnata and P. cincinnata var. BRS Sertão Forte grouped by the influence of the fatty acids, sucrose, flavones (isoorientin and vitexin-2- O "-xyloside), and trigonelline. The varieties of P. setacea, and P. cincinnata are chemically equivalent to the original Passiflora species. However, the PCA analysis showed that the genetic variety of P. alata occupied a different position in the scores plot provoked mainly by the presence of oleanolic acid-3-sophoroside. The 1 H NMR metabolic profile can be efficient for quality control evaluation, and can contribute to the investigation of new alternatives for official Passiflora herbal medicines.