A rapid and sensitive lateral flow immunoassay (LFIA) for the detection of gluten in foods. (1st September 2021)

• Record Type: Journal Article
• Authors: Hnasko, Robert M.; Jackson, Eric S.; Lin, Alice V.; Haff, Ronald P.; McGarvey, Jeffery A.
• Is Part Of: Food chemistry. Volume 355(2021)
• Publication Date: 2021-09-01
• Subjects: Gluten -- Gliadin -- Food allergen -- Lateral Flow Immunoassay (LFIA) -- Monoclonal antibodies -- Biosensor; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.129514
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Monoclonal antibodies for sensitive and selective gluten detection. Rapid gluten detection (<5 min) by lateral flow immunoassay. A single-use biosensor for easy gluten testing of foods. Abstract: The gluten protein found in a variety of cereal grains is a food allergen that can elicit a spectrum of immuno-inflammatory responses in people. Consumer awareness has prompted changes in food labeling requirements, expanded gluten-free food product availability and increased demand for effective gluten testing methodologies. To meet the challenges associated with gluten testing from diverse and complex foods we developed a lateral flow immunoassay (LFIA) using a pair of novel gliadin monoclonal antibodies (MAbs). Using a visual gold reporter, we show sensitive gluten detection (150 ng/mL) from complex food substrates using a fast (<5 min) and easy testing methodology. In this report we characterize the binding properties of a cohort of newly generated gliadin monoclonal antibodies suitable for gluten detection using multiple assay formats and introduce a novel plug- n -play test strip platform with integrated test components in a single-use format.