Influence of different factors on biofilm formation of Listeria monocytogenes and the regulation of cheY gene. (November 2020)

• Record Type: Journal Article
• Authors: Fan, Yun; Qiao, Jiaju; Lu, Zhaoxin; Fen, Zhiyang; Tao, Yang; Lv, Fengxia; Zhao, Haizhen; Zhang, Chong; Bie, Xiaomei
• Is Part Of: Food research international. Volume 137(2020)
• Publication Date: 2020-11
• Subjects: Listeria monocytogenes -- Biofilm, environmental factors -- Cell properties -- Chemotaxis gene cheY; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2020.109405
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Temperature has the greatest effect on Listeria monocytogenes biofilm formation. Effects of different factors on biofilm formation are strain-dependent. Motility and hydrophobicity are positively related to biofilm-forming capacity. cheY gene positively regulates biofilm formation via motility and hydrophobicity. Abstract: In a food-processing environment, bacterial cells often adhere to surfaces and form biofilms to protect themselves from external adverse influences. Our study aimed to identify the influence of environmental factors and cell properties on Listeria monocytogenes biofilm formation. Biofilm formation was quantified through measuring the optical density at 590 nm (OD590 nm ) after crystal violet staining. Neutral pH and 37oC were beneficial for biofilm formation whereas the influence of glucose (0.0–1.0%) and sodium chloride (0.0–1.0%) were strain-dependent. In general, the addition of sodium chloride and glucose increased biofilm formation in most strains compared to that in controls with no sodium chloride or glucose added. Bacteria with strong biofilm-forming capacity always produced large amounts of biofilm in most instances. Biofilm formation positively correlated with the cell surface hydrophobicity and motility but was independent from planktonic cell growth. The expression of flagella-related flaA, motB, and the two-component chemotactic system cheA/Y genes in biofilm cells increased compared to that in planktonic cells. Meanwhile, a cheY knockout mutant was constructed, and decreased biofilm-formation ability along with reduced cell-surface hydrophobicity were found in the non-motile mutant. Furthermore, the cheY knockout mutant showed no change in growth, and pH susceptibility compared to that in the wild-type strain.