Attenuation of protein glycation by functional polyphenolics of dragon fruit (Hylocereus polyrhizus); an in vitro and in silico evaluation. (February 2021)

• Record Type: Journal Article
• Authors: Ravichandran, Guna; Lakshmanan, Dinesh Kumar; Murugesan, Selvakumar; Elangovan, Abbirami; Rajasekaran, Namakkal S.; Thilagar, Sivasudha
• Is Part Of: Food research international. Volume 140(2021)
• Publication Date: 2021-02
• Subjects: Non-enzymatic glycation -- Advanced glycation end products -- Processed foods -- Oxidative stress -- Inflammation -- Nutraceuticals; AP Amadori products -- AGEs advanced glycation end products -- OS oxidative stress -- CVD cardiovascular diseases -- ROS Reactive oxygen species -- RCS reactive carbonyl species -- MDA melondialdehyde -- RAGE Receptor for AGEs -- NFκB Nuclear factor kappa-light-chain-enhancer of activated B cells; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2020.110081
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: H. polyrhizus has exhibited efficient anti-glycative potential against food AGEs in vitro assays. UPLC-ESI-MS/MS analysis has revealed bioactive flavonoid and other phenolic compounds from H. polyrhizus . Evaluated in silico interaction between 4-prenylresveratrol, vicenin, and luteolin and target protein RAGE. Anti-glycative mechanism of identified polyphenolics was correlated with their chemical structure. Abstract: Chronic hyperglycemia and oxidative stress promote non-enzymatic glycation that leads to the production of advanced glycation end products (AGEs). AGEs casue significant damage to physiological proteins which result in several complications. The scenario also corresponds to the chronic consumption of a diet rich in AGEs. Despite understanding these mechanisms at the molecular level, the discovery of new drugs for these complications is under progress. Natural compounds might have great therapeutic potential for treating glycative consequences. In view of this, the study aimed to evaluate fruit extracts of Hylocereus polyrhizus towards determining its phenolics and flavonoid contents, as well as assessing it's in vitro antiglycative potential through the use of multistage glycation markers (early, intermediate and end stage products of β-aggregation) in sugar–protein model. In vitro hypoglycemic activity of H. polyrhizus extracts was evaluated through α-amylase and α glucosidase inhibitory activities. In vitro antioxidant potential of the fruit extracts was also examined against different free radical types including DPPH and ABTS. Among the different in vitro assays performed, methanolic and acetone extracts of the fruit, with higher phenolics and flavonoid content, have exerted significant antiglycation and antioxidant activities than other extracts namely aqueous, ethanol, hydro-ethanol, hydro-methanol, and petroleum ether. Ultra-Performance Liquid Chromatography coupled with Electrospray Ionization Mass Spectrometry (UPLC-ESI-MS/MS) analysis was employed to identify active polyphenolics that may be responsible for the antiglycative potential of H. polyrhizus . The analysis revealed some high-profile compounds that have well documented for their therapeutic benefits. Additionally, In silico analysis also showed the possible connection between identified compounds and mechanisms of action. 4- Prenylresveratrol, Vicenin, and Luteolin had observed as effectively interact with target protein in molecular docking analysis. This suggests H. polyrhizus as a good source of anti-glycation and antioxidants that may have potential applications for the treatment and prevention of glycation associated diabetic and aging complications.