Comparative transcriptomic analysis of cantaloupe melon under cold storage with ozone treatment. (February 2021)

• Record Type: Journal Article
• Authors: Zhang, Xiaojun; Tang, Ning; Zhang, Huijie; Chen, Cunkun; Li, Li; Dong, Chenghu; Cheng, Yongqiang
• Is Part Of: Food research international. Volume 140(2021)
• Publication Date: 2021-02
• Subjects: Ozone -- Cantaloupe -- Transcriptome -- RNA sequencing -- Gene ontology; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2020.109993
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Ozone treatment can delay postharvest cantaloupe decay and improve its intrinsic quality. Transcriptomic analysis was performed to reveal the mechanism of cantaloupe in response to ozone treatment. Differentially expressed genes were related to ethylene metabolism, flavonoid metabolism and pectin metabolism. Abstract: Ozone treatment was found to delay the postharvest cantaloupe melon decay and improve its intrinsic quality during the cold storage. The transcriptomes of cantaloupe peel and pulp in response to ozone treatment were investigated to reveal the mechanisms using a high-throughput RNA sequencing approach. Results showed that 570 and 313 differentially expressed genes were identified in peel and pulp, respectively. According to these identified genes, the gene ontology and pathway enrichment analysis indicated that the ozone treatment could maintain the firmness of the cantaloupe by changing pectin metabolites and reduction of the ethylene production by regulating relevant genes especially in the peel. The total flavonoid content changes in peel and pulp related to the regulation of phenylalanine ammonia lyase, 4-coumarate-CoA ligase and P450 family genes which further leading to the inhibition of phenylalanine metabolic pathway in peel but promotion of secondary metabolism in pulp. The qRT-PCR results were in accordance with our RNA sequencing results which validated the conclusions. The present study for the first time reveals the mechanism of cantaloupe in response to ozone treatment at a transcriptome level which is of importance for cantaloupe storage.