Differences in milk fat globule membrane proteins among Murrah, Nili-Ravi and Mediterranean buffaloes revealed by a TMT proteomic approach. (January 2021)

• Record Type: Journal Article
• Authors: Li, Shanshan; Yang, Yongxin; Chen, Chen; Li, Ling; Valencak, Teresa G.; Ren, Daxi
• Is Part Of: Food research international. Volume 139(2021)
• Publication Date: 2021-01
• Subjects: Buffalo -- Milk fat globule membrane protein -- Quantitative proteome -- Tandem mass tag; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2020.109847
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: MFGM protein components among buffalo breeds identified by a TMT proteomic approach. Newly MFGM proteins identified and exert antioxidant and immunoregulatory activities. Differences in MFGM proteins among buffalo breeds present by multivariate analysis. TLR2 in Mediterranean, ERP29 in Nili-Ravi, UMOD in Murrah have higher abundance. Abstract: Milk fat globule membrane (MFGM) proteins contribute to human nutrition and health. However, differences in MFGM proteome among the buffalo breeds are unknown. In this study, MFGM proteins from Murrah, Nili-Ravi and Mediterranean buffaloes were identified using a tandem mass tag proteomic approach to characterize the components and their potential activities. A total of 1258 MFGM proteins were identified and 103 differentially expressed proteins were found in the studied buffalo breeds. Of these, toll-like receptor 2 had higher abundance in Mediterranean milk; endoplasmic reticulum resident protein 29 had higher levels in Nili-Ravi milk; uromodulin had higher abundance in Murrah milk. A number of 781 newly proteins in buffalo milk exerted antioxidant, antibacterial, and anti-inflammatory activities. Our findings reveal the component complexity and functional diversity of MFGM proteins in different buffalo breeds. Thus, our data may path the way to promote raw buffalo milk as a healthy and functional dairy product.