Isolation and functional characterization of hemp seed protein-derived short- and medium-chain peptide mixtures with multifunctional properties for metabolic syndrome prevention. (January 2023)

• Record Type: Journal Article
• Authors: Cerrato, Andrea; Lammi, Carmen; Laura Capriotti, Anna; Bollati, Carlotta; Cavaliere, Chiara; Maria Montone, Carmela; Bartolomei, Martina; Boschin, Giovanna; Li, Jianqiang; Piovesana, Susy; Arnoldi, Anna; Laganà, Aldo
• Is Part Of: Food research international. Volume 163(2023)
• Publication Date: 2023-01
• Subjects: Peptidomics -- Size-exclusion chromatography -- LC-HRMS -- ACE -- DPP-IV -- HMGCoAR; ACE angiotensin converting enzyme -- ANOVA analysis of variance -- AP apical -- BCA Bicinchoninic Acid Protein Assay -- BL basolateral -- DPP-IV dipeptidyl peptidase IV -- DPPH 2, 2-Diphenyl-1-picrylhydrazyl -- EFSA European Food Safety Authority -- FRAP Ferric Reducing Antioxidant Power -- GCB graphitized carbon black -- HMGCoAR 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase -- LDL low-density lipoprotein -- LDLR low-density lipoprotein receptor pathway -- M medium-chain peptide mixture of the hemp seed hydrolysate -- MetS Metabolic Syndrome -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide -- PCSK9 Proprotein convertase subtilisin/kexin type 9 -- PepT1 peptide transport 1 -- RYR red yeast rice -- S short-chain peptide mixture of the hemp seed hydrolysate -- SDC sodium deoxycholate -- SEC size exclusion chromatography -- T total hemp seed hydrolysate -- TFA trifluoroacetic acid -- UFM ultrafiltration membrane -- UHPLC-HRMS ultra-high-performance liquid chromatography - high-resolution mass spectrometry; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.112219
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Both SEC and UFM were applied for isolating short-chain peptides. More than 500 hemp seed short-chain peptides were identified. Hemp seed short-chain peptides exert multifunctional activities. Hemp seed peptides may be used for developing nutraceutical and functional foods. Abstract: This study aims to obtain a valuable mixture of short-chain peptides from hempseed as a new ingredient for developing nutraceutical and functional foods useful for preventing metabolic syndrome that represents the major cause of death globally. A dedicated analytical platform based on a purification step by size exclusion chromatography or ultrafiltration membrane and high-resolution mass spectrometry was developed to isolate and comprehensively characterize short-chain peptides leading to the identification of more than 500 short-chain peptides . Our results indicated that the short-chain peptide mixture was about three times more active than the medium-chain peptide mixture and total hydrolysate with respect to measured inhibition of the angiotensin-converting enzyme. The short-chain peptide mixture was also two times more active as a dipeptidyl peptidase IV, and twofold more active on the cholesterol metabolism pathway through the modulation of low-density lipoprotein receptor.