Effect of oxidation and in vitro intestinal hydrolysis on phospholipid toxicity towards HT29 cell line serving as a model of human intestinal epithelium. (January 2023)

• Record Type: Journal Article
• Authors: Parchem, Karol; Baranowska, Monika; Kościelak, Anna; Kłosowska-Chomiczewska, Ilona; Domingues, M. Rosário; Macierzanka, Adam; Bartoszek, Agnieszka
• Is Part Of: Food research international. Volume 163(2023)
• Publication Date: 2023-01
• Subjects: Oxidized phospholipids -- Phospholipid hydrolysis -- Pancreatic phospholipase A2 -- Bile salts -- Intestinal oxidative stress; BS bile salt -- CLD cytosolic lipid droplet -- ER endoplasmic reticulum -- FAME fatty acid methyl ester -- GPx2 gastrointestinal glutathione peroxidase 2 -- GSH glutathione -- GSSG glutathione disulfide -- LOH lipid hydroxide -- LOOH lipid hydroperoxide -- LOP lipid oxidation product -- LOX lipoxygenase -- LPL lysophospholipid -- MDA malondialdehyde -- oxPL oxidized phospholipid -- PC phosphatidylcholine -- PDI polydispersity index -- PE phosphatidylethanolamine -- PL phospholipid -- PLA2 phospholipase A2 -- PV peroxide value -- RNS reactive nitrogen species -- ROS reactive oxygen species -- SIF simulated intestinal fluid -- IEC intestine epithelial cell -- TAG triacylglycerol; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.112227
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Oxidation of hen egg yolk PLs decreased the rate of their hydrolysis catalysed by PLA2. PLA2 inhibition was caused by low-molecular aldehydes, including hexanal and ( E )-2-nonenal. PL oxidation products after hydrolysis exhibited the highest cytotoxicity towards HT29 cells. Native and oxidized PLs, both before and after hydrolysis, decreased BS-induced DNA strand breaks. Native and oxidized PL hydrolysis increased neutral lipid accumulation in CLDs in HT29 cells. Abstract: Oxidation of food-derived phospholipids (PLs) can influence nutrient digestion and induce oxidative stress in gastrointestinal epithelium. In this study, hen egg yolk PL fraction was used to evaluate the effect of lipoxygenase (LOX)-induced PL oxidation on the rate of PL hydrolysis catalyzed by pancreatic phospholipase A2 (PLA2 ) in the presence of bile salts (BSs). Then, PL/BS solutions containing native or oxidized PLs were used in in vitro intestinal digestion to assess the effect of PL oxidation and hydrolysis on the toxicity towards HT29 cell line. Based on the obtained results, we suggest that hexanal and ( E )-2-nonenal, formed by the decomposition of PL hydroperoxides, inhibited PLA2 activity. The cell exposure to simulated intestinal fluid (SIF) containing BSs decreased HT29 cell viability and significantly damaged cellular DNA. However, the genotoxic effect was reversed in the presence of all tested PL samples, while the protective effect against the BS-induced cytotoxicity was observed for native non-hydrolyzed PLs, but was not clearly visible for other samples. This can result from an overlap of other toxic effects such as lipotoxicity or disturbance of cellular redox homeostasis. Taking into account the data obtained, it was proposed that the PLA2 activity decline in the presence of PL oxidation products may be a kind of protective mechanism against rapid release of oxidized FAs characterized by high cytotoxic effect towards intestinal epithelium cells.