Acidic hydrolyzed xylo-oligosaccharides bioactivity on the antioxidant and immune activities of macrophage. (January 2023)

• Record Type: Journal Article
• Authors: Liu, Xinlu; Cao, Rou; Xu, Yong
• Is Part Of: Food research international. Volume 163(2023)
• Publication Date: 2023-01
• Subjects: Xylo-oligosaccharides -- Acetic acid hydrolysis -- Purification -- Antioxidant activity -- Immune activity -- Macrophage; XOS xylo-oligosaccharides -- PXOS Purified-XOS -- CXOS Crude-XOS -- TNF-α Tumor necrosis factor-α -- SOD Superoxide dismutase -- MDA Malondialdehyde -- FBS Fetal bovine serum -- DMEM Dulbecco's modified Eagle's medium -- LPS Lipopolysaccharide -- DMSO Dimethyl sulfoxide -- CCK-8 Cell counting kit -- NO Nitric oxide -- IL-6 Interleukin-6 -- IL-1β Interleukin-1β -- G.oxydans Gluconobacter oxydans -- BMED Bipolar membrane electrodialysis; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.112152
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: The purification process helps to improve the antioxidant and immune activities of XOS from acidolysis slurry. Purified-XOS had a more substantial effect on enhancing anti-oxidation. Purified-XOS was significantly higher on the secretion of cytokines. Abstract: Xylo-oligosaccharides (XOS) prepared by the acetic acid hydrolysis of corncob were adulterated with many impurities including pigments, salts, and monosaccharides. Monosaccharides, acids, and most of the pigment were removed by a combination of decolorization, bipolar membrane electrodialysis and catalysis by Gluconobacter oxydans . These steps retain 90% of XOS in the acidolysis slurry. In this study, the effects of purified-XOS (PXOS) and crude XOS (CXOS) on the antioxidant and immune activities of macrophage were compared to verify the bioactivity of acidic hydrolyzed XOS, mainly focusing on the benefits of the purification process. PXOS was more effective in increasing superoxide dismutase activity and reducing malondialdehyde content, and thus had more potent antioxidant activity. In addition, PXOS could more efficiently promote the secretion of tumor necrosis factor-α, interleukin-6, nitric oxide, and interleukin-1β by macrophage. All these data, suggest that the purification process contributed to improve the immunomodulatory activity of XOS from acidolysis slurry.