Gastrointestinal digestion products of shrimp (Penaeus vannamei) proteins retain an allergenic potential. (December 2022)

• Record Type: Journal Article
• Authors: Liu, Yao; Lin, Songyi; Liu, Kexin; Wang, Shan; Sun, Na
• Is Part Of: Food research international. Volume 162(2022)Part A
• Publication Date: 2022-12
• Subjects: Penaeus vannamei -- Food allergy -- Simulated digestion -- BALB/c mice model; GI Gastrointestinal -- FAO Food and Agriculture Organization of the United Nations -- EFSA European Food Safety Authority -- WHO World Health Organization -- CAC Codex Alimentarius Commission -- mMCP-1 Mouse mast cell protease-1 -- SDS-PAGE Sodium dodecyl sulfate-polyacrylamide gel electrophoresis -- SGF Simulated gastric fluid -- SIF Simulated intestinal fluid -- SPE Shrimp protein extract -- SGD Simulated gastric digests -- SID Simulated intestinal digests -- SGID Simulated gastrointestinal digests -- TCA Trichloroacetic acid -- ELISA Enzyme-linked immunosorbent assay -- PBS Phosphate buffer saline -- BSA Bovine serum albumin -- H&E Hematoxylin and eosin -- SEM Standard error of mean -- TM Tropomyosin -- MHC Myosin heavy chain -- AK Arginine kinase -- MLC Myosin light chain; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111916
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: The low molecular protein formed by digestion still has the allergenic capacity. Gastric digestion reduced the allergenic potential of shrimp protein. Gastrointestinal digestion maintained the allergenicity of shrimp protein. Abstract: The allergenic potential of shrimp ( Penaeus vannamei ) proteins during gastrointestinal (GI) digestion is currently unclear. In this study, the role of gastric, intestinal, and GI digestion on the sensitizing and eliciting capacity of shrimp proteins was investigated using a BALB/c mice model. Gastric digestion products of shrimp proteins produced lower specific IgE/IgG1, histamine and mMCP-1 levels and significantly reduced the proportion of degranulated mast cells as compared to the shrimp protein extract; furthermore, no histopathological changes were observed in the lung, spleen and jejunum. Conversely, their GI digestion products caused high specific IgE/IgG1 levels, mast cells degranulation and severe allergic symptoms in the mice, similar to those in the shrimp protein extract group. These results may be due to the masking or exposure of shrimp epitopes during digestion. Consequently, the GI digestion products of shrimp proteins retained an allergenic potential, although gastric digestion products showed a lowering effect.