Application of metabolomics to explore the automatic oxidation process of hazelnut oil. (December 2022)

• Record Type: Journal Article
• Authors: Gao, Yan; Cui, Nana; Liu, Jing; Ma, Qinghua; Zhao, Tiantian; Yang, Zhen; Zhao, Hongfei; Zhang, Bolin; Liang, Lisong
• Is Part Of: Food research international. Volume 162(2022)Part A
• Publication Date: 2022-12
• Subjects: Cold-pressed hazelnut oil -- Automatic oxidation -- Metabolomics -- Free radical -- UPLC-QE/MS -- Metabolic pathway; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111888
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Cold pressed hazelnut oil was accelerated oxidized at 60℃. The color of oil was changed with the deepening of oxidation. The free radical intensity was a good indicator of lipid oxidation level, which matched with lipid color change. The UPLC--EQ/MS method was used to track and monitor the oxidative metabolites of hazelnut oil, while the oxidative metabolic pathway of hazelnut oil was analyzed by bioinformatics method. Application of metabonomics to elucidate the material basis of oxidative metabolism of hazelnut oil. Highlights: Deep oxidation results in complete discoloration of hazelnut oil. Free radical intensity is a good indicator of the oxidation degree of hazelnut oil. Total of 1010 metabolites in 12 super classes were detected in fresh hazelnut oil. Linoleic acid metabolism and sphingolipid metabolism are the most important metabolic pathways in hazelnut oil oxidation process. Application of metabonomics to elucidate the material basis of oxidative metabolism of hazelnut oil. Abstract: The oxidation metabolites of hazelnut oil are complex and vary with different degrees of oxidation. At present, few studies have investigated the change law of metabolites during the oxidation process of hazelnut oil. In this study, ultrahigh-performance liquid chromatography tandem Q-Exactive high-resolution mass spectrometry (UPLC-QE/MS) was used to analyze the differential metabolites resulting from the oxidation of cold-pressed hazelnut oil during storage for 40 days with accelerated oxidation. The oxidation level of cold-pressed hazelnut oil was evaluated by monitoring the free radical relative strength during accelerated oxidation. A total of 1010 metabolites in 12 super classes were detected in fresh hazelnut oil. Based on multivariate statistical analysis of all metabolites and the change law of free radicals in hazelnut oil, it was found that hazelnut oil enters the deep oxidation stage after accelerated oxidation for 30 days. A statistical analysis of differential metabolites and metabolic pathways was also carried out. The metabolite map obtained in this study can further distinguish hazelnut oil with different degrees of oxidation. Bioinformatics analysis indicated that linoleic acid metabolism and sphingolipid (SP) metabolism are the most important metabolic pathways in the entire oxidation process. These results provide a basis for better understanding the composition of hazelnut oil metabolites with different oxidation levels, identifying markers for oxidation level evaluation and analyzing the oxidative metabolism mechanism of hazelnut oil. This study provides new resources and new ideas for studying methods to prolong the shelf life of edible oils.