Red algae-derived isofloridoside activates the sweet taste receptor T1R2/T1R3. (December 2022)

• Record Type: Journal Article
• Authors: Akishino, Miku; Aoki, Yuko; Baba, Hajime; Asakawa, Makio; Hama, Yoichiro; Mitsutake, Susumu
• Is Part Of: Food bioscience. Volume 50:Part B(2022)
• Publication Date: 2022-12
• Subjects: GPCR -- Taste receptor -- T1R2 -- T1R3 -- Floridoside -- Pyropia yezoensis; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2022.102186
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: Isofloridoside (IF) is a naturally occurring galactosylglycerol compound with a refreshing sweet taste. It is found in red algae, where it acts as a photosynthetic intermediate. However, the mechanism by which IF elicits sweetness has not been determined. In this study, we investigate the interaction between IF and the sweet taste receptor T1R2/T1R3. We show that IF causes an increase in intracellular Ca 2+ concentration and an increase in Erk phosphorylation in T1R2/T1R3-expressing HEK293T cells, indicating that IF interacts with T1R2/T1R3. IF also activates endogenously expressed T1R2/T1R3 in mouse small intestinal endocrine L-cells, and promotes the secretion of the incretin glucagon-like peptide-1 (GLP-1). In silico docking simulations of IF and T1R2/T1R3 predict that IF forms hydrogen bonds with Tyr103 in the extracellular Venus flytrap domain of T1R2. This result is consistent with the binding mode of many other sweet-tasting molecules. We found that IF have the potential as a new alternative sweetener. Graphical abstract: Image 1