Targeting the gut microbiota to investigate the mechanism of Lactiplantibacillus plantarum 1201 in negating colitis aggravated by a high-salt diet. (December 2022)

• Record Type: Journal Article
• Authors: Chen, Shufang; Ren, Zhongyue; Huo, Yalan; Yang, Wanyu; Peng, Lingling; Lv, Huihui; Nie, Lijuan; Wei, Hua; Wan, Cuixiang
• Is Part Of: Food research international. Volume 162(2022)Part A
• Publication Date: 2022-12
• Subjects: High salt diet -- Intestinal flora -- Ulcerative colitis -- Lactiplantibacillus plantarum 1201 -- Alpha-tocopherol -- D-mannose; IBD inflammatory bowel disease -- DSS dextran sulfate sodium -- HSD high salt diet (4% NaCl) -- ND Normal salt diet -- NDS Normal salt diet and drink 2.5% DSS water for five days -- HSDS high salt diet and DSS for five days -- HSDS-1201 high salt diet, DSS for five days and intragastric gavage of L. plantarum 1201 for 1 week -- DSS Drink 2%DSS water for one week -- V-DSS 2%DSS water for 1 week and 0.1% α-T diet for 2 weeks. D-DSS, 2%DSS water for 1 week and 20% D-Mannose for 2 weeks -- VD-DSS 2%DSS water for 1 week while 0.01% α-T diet, 20% D-Mannose gavage for 2 weeks. UC, ulcerative colitis -- CD crohn's disease -- H&E hematoxylin and Eosin -- ZO-1 zonula occludens-1 -- OTU operational taxonomic units -- UHPLC-Q-TOF-MS Ultra-performance liquid chromatography-Q-TOF-mass spectrometry -- PCA Principal component analysis -- KEGG Kyoto Encyclopedia of Genes and Genomes -- IL-6 Interleukin 6 -- TGF-β transforming growth factor-β -- IFN-γ Interferon γ -- IL-22 Interleukin 12 -- IL-1β Interleukin 1β -- IL-10 Interleukin 10 -- IL-17A Interleukin 17A -- TNF-α tumor necrosis factor-α -- NF-κB nuclear factor kappa-B -- IκB-α inhibitor of NF-κB; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.112010
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: HSD aggravated colitis by disturbing gut microbiota and activating NF- κ B pathway. L. plantarum 1201 alleviated colitis through partially restoring intestinal flora. 1201 increased serum levels of α-T and D-mannose through intestinal flora metabolism. α-T and D-mannose alleviated colitis through modulating intestinal immune function. Abstract: High-salt diet (HSD) affects the composition and function of the intestinal microbiota and cause health problems. This study confirmed that HSD aggravates dextran sulphate sodium (DSS)-induced colitis by changing the relative abundance of the gut microbiota, activating the NF-κB pathway, and up-regulating the mRNA levels of inflammatory factors. We explored the effect of L. plantarum 1201 in negating DSS-induced ulcerative colitis, which is aggravated by HSD for the first time. Results show that L. plantarum 1201 rebuilt the balance of intestinal flora by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundance of Bifidobacterium, Lactobacillus and butyric-producing bacteria. Moreover, L. plantarum 1201 inhibited the up-regulation of inflammatory cytokines (e.g., IL-1β, TNF-α, IL-6, IL-22, and IFN-γ) mRNA levels, increased colonic tight junction protein (ZO-1, ocludin, and claudin-3) expression, and increased serum levels of beneficial metabolites, including alpha-tocopherol (α-T) and D-mannose. By reconstructing an animal model of colitis, we further discovered that α-T and D-mannose inhibited the NF-κB pathway, improved tissue injury, and decreased the expression of pro-inflammatory cytokines (e.g., IL-1β, TNF-α, and IL-6). This study proves for the first time that L. plantarum 1201 attenuates high-salt-aggravated colitis by increasing the serum concentrations of endogenic D-mannose in mice serum and inhibiting the consumption of α-T through intestinal flora. Therefore, regulating the gut microbiota is a potential treatment for high-salt-aggravated colitis.