Walnut-derived peptides ameliorate d-galactose-induced memory impairments in a mouse model via inhibition of MMP-9-mediated blood–brain barrier disruption. (December 2022)

• Record Type: Journal Article
• Authors: Dang, Qiao; Wu, Dan; Li, Yanru; Fang, Li; Liu, Chunlei; Wang, Xiyan; Liu, Xiaoting; Min, Weihong
• Is Part Of: Food research international. Volume 162(2022)Part A
• Publication Date: 2022-12
• Subjects: Walnut-derived peptides -- d-galactose -- Cognitive impairment -- Blood-brain barrier -- Tight junction -- MMP-9; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.112029
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Walnut peptides ameliorate d -galactose-induced memory impairments in a mouse model. Amelioration is due to inhibition of MMP-9-mediated blood–brain barrier disruption. TW-7 maintains BBB integrity by inhibiting the expression and activity of MMP-9. Abstract: Disruption of the blood–brain barrier (BBB) has been observed in several neurological diseases. This study explored the protective effect of the walnut-derived peptide TWLPLPR (TW-7) against d -galactose (d -gal)-induced cognitive disturbance in mice and its potential protection mechanism in β -amyloid 25–35 (A β 25-35 )-injured bEnd.3 cells. TW-7 improved the learning ability and memory of the cognitive impairment mice. Transmission electron microscopy showed that the BBB integrity in the hippocampus was restored; while immunofluorescence analysis and western blotting indicated that the protection of BBB integrity was associated with increased expression levels of tight junction (TJ) proteins. In A β 25-35 -damaged bEnd.3 cells, treatment with a matrix metalloproteinase 9 (MMP-9) activator and inhibitor confirmed that TW-7 partially reduced MMP-9 expression and increased zonula occludin-1 (ZO-1) and Claudin-5 expression. Furthermore, TW-7 reduced MMP-9 levels by forming stable complexes with it and by inhibiting the NF- κ B p65/iNos pathway. These data suggested that TW-7 maintains BBB integrity by inhibiting the expression and activity of MMP-9, and TW-7 improves learning and memory ability in mice.