Rapid quality control of black truffles using Direct Analysis in Real Time Mass Spectrometry and Hydrophilic Interaction Liquid Chromatography Mass Spectrometry. (1st March 2023)

• Record Type: Journal Article
• Authors: Losso, Klemens; Wörz, Hannah; Kappacher, Christoph; Huber, Susanne; Jakschitz, Thomas; Rainer, Matthias; Bonn, Günther K.
• Is Part Of: Food chemistry. Volume 403(2023)
• Publication Date: 2023-03-01
• Subjects: ACN Acetonitrile -- DART Direct Analysis in Real Time -- ESI Electrospray Ionization -- FA Formic acid -- GC–MS Gas Chromatography Mass Spectrometry -- HILIC Hydrophilic Interaction Liquid Chromatography -- HPLC High Performance Liquid Chromatography -- H-PTFE Hydrophilized polytetrafluoroethylene -- LD Linear Discriminant -- LDA Linear Discriminant Analysis -- MALDI-TOF-MS Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry -- NIR Near Infrared Spectroscopy -- PC Principal Component -- PCA Principal Component Analysis -- QTOF Quadrupole Time of Flight -- QQQ Triple Quadrupole; Ambient MS -- HILIC-ESI-MS -- Tuber melanosporumVittad. -- Tuber aestivumVittad. -- Tuber uncinatumChatin -- Tuber indicumCooke etMassee; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134418
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Rapid and easy classification of truffle samples with DART-MS and HILIC-MS. Ability to differentiate the species of black truffles within seconds. Excellent prediction accuracies of up to 99% with DART-MS. Perfect prediction accuracies of 100% with HILIC-MS. Reduced time consumption and increased robustness of DART-MS compared to HILIC-MS. Abstract: This work is the first to describe the application of Direct Analysis in Real Time Mass Spectrometry for rapid and simple quality control of black truffles. Multivariate analyses (PCA and LDA) of mass spectra provided highly efficient models for the discrimination of four truffle species, namely Tuber melanosporum Vittad., Tuber aestivum Vittad., Tuber uncinatum Chatin and Tuber indicum Cooke et Massee . Full-cross validations showed prediction accuracies up to 99%. Hydrophilic Interaction Liquid Chromatography Mass Spectrometry was used as a reference method and the results of both methods were compared to each other. Multivariate models of the chromatograms also showed excellent results with prediction accuracies of 100%. In a direct comparison of methods, Direct Analysis in Real Time Mass Spectrometry showed minimally lower prediction accuracies, but was significantly faster, more robust and easier to use, making it an excellent choice for rapid quality control of black truffles.