Dietary l-carnitine supplementation recovers the increased pH and hardness in fillets caused by high-fat diet in Nile tilapia (Oreochromis niloticus). (15th July 2022)

• Record Type: Journal Article
• Authors: Zhang, Zhi-Yong; Limbu, Samwel M.; Zhao, Si-Han; Chen, Li-Qiao; Luo, Yuan; Zhang, Mei-Ling; Qiao, Fang; Du, Zhen-Yu
• Is Part Of: Food chemistry. Volume 382(2022)
• Publication Date: 2022-07-15
• Subjects: Aquaculture -- Dietary lipid -- Lipid catabolism -- Flesh quality -- Myofibers -- pH value; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132367
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: High-fat diet (HFD) feeding in Nile tilapia increased flesh hardness and pH value. HFD feeding induced muscle atrophy and reduced myofiber diameter in Nile tilapia. Dietary l -carnitine supplementation in HFD restored flesh hardness through reducing ER stress and apoptosis in myofibers. Dietary l -carnitine supplementation in HFD restored flesh pH value through elevating lipid catabolism and regulating lactic acid production. Dietary l -carnitine supplementation is an efficient method to regulate flesh quality in fish. Abstract: The wide use of high-fat diet (HFD) causes negative effects on flesh quality in farmed fish. l -carnitine, a lipid-lowering additive, enhances mitochondrial fatty acid β-oxidation. However its roles in alleviating the effects of HFD on flesh quality in fish are unknown. We fed Nile tilapia with medium-fat diet (MFD, 6% dietary lipid), high-fat diet (HFD, 12% dietary lipid) and HFCD supplemented with l -carnitine (HFCD + 400 mg/kg l -carnitine) for 10 weeks. The HFD-fed fish had higher fat deposition, pH value, myofiber density and flesh hardness than those fed on MFD. However, feeding the fish with the HFCD improved lipid catabolism, which increased significantly lactic acid content and myofiber diameter in muscle, thus reduced pH and hardness values. HFCD also reduced endoplasmic reticulum stress and myofiber apoptosis caused by HFD in the fish. Our study suggests that dietary l -carnitine supplementation alleviates the negative effects of HFD on flesh quality of farmed fish.