Absorption and transport of myofibrillar protein-bound Nɛ-(carboxymethyl)lysine in Caco-2 cells after simulated gastrointestinal digestion. (November 2022)

• Record Type: Journal Article
• Authors: Ye, Mengyu; Qi, Xiaomin; Ren, Xiaomin; Quan, Wei; Xu, Huaide; Zeng, Maomao; Chen, Jie; Li, Mei
• Is Part Of: Food research international. Volume 161(2022)
• Publication Date: 2022-11
• Subjects: Myofibrillar protein-bound Nɛ-(carboxymethyl)lysine -- Simulated gastrointestinal digestion -- Absorption -- Transport -- Caco-2 cell; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111870
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: MP-bound CML could be absorbed by 35.06% into Caco-2 cells after simulated gastrointestinal digestion. The absorption proportion of CML in MP-bound CML hydrolysates with MW less than 5 kDa accounted for 97.09 % of the total CML accumulation levels. MP-bound CML could be transported across Caco-2 cells through paracellular pathway, transcytosis route and active route via PepT-1. Abstract: This study aimed to investigate the absorption and transport of myofibrillar protein-bound N ɛ -(carboxymethyl)lysine (MP-bound CML) in Caco-2 cells after simulated gastrointestinal digestion. Four kinds of MP-bound CML hydrolysates with molecular weights (MWs) less than 1 kDa, 1–3 kDa, 3–5 kDa and greater than 5 kDa, were obtained by ultrafiltration; their absorption and transport were studied in Caco-2 cells. Peptide-bound CML in hydrolysates with MWs less than 1 kDa was absorbed by 6.58 % and might transport across Caco-2 cells monolayer through paracellular pathway; peptide-bound CML in hydrolysates with MWs 1–3 kDa was absorbed by 12.8 % and might transport across Caco-2 cells monolayer through paracellular pathway and transcytosis route; peptide-bound CML in hydrolysates with MWs 3–5 kDa was absorbed by 14.66 % and might be through active route via PepT-1 transport across Caco-2 cells monolayer; whereas protein-bound CML in hydrolysates with MWs greater than 5 kDa was only absorbed by 1.02 %, which was hardly transported into Caco-2 cells. In conclusion, MP-bound CML could be absorbed by 35.06 % into Caco-2 cells after simulated gastrointestinal digestion and is transported across Caco-2 cells through paracellular pathway, transcytosis route and active route via PepT-1.