Nanomaterials: An Efficient Support to Immobilize Microbial α–Amylases for Improved Starch Hydrolysis. (20th July 2022)

• Record Type: Journal Article
• Authors: Kikani, Bhavtosh A.; Suthar, Sadikhusain; Joshi, Disha
• Is Part Of: Stärke. Volume 74:Number 9/10(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-07-20
• Subjects: immobilization -- microbial amylase -- nanomaterials -- reusability -- stability; Starch -- Periodicals; 572.566
• DOI: 10.1002/star.202200093
• Languages: English
• ISSNs: 0038-9056

Abstract

Abstract: Microbial enzymes have contributed significantly to the development of biotech based industries over the recent past. Importantly, the microbial α‐amylases share a major market along with proteases. Starch gets hydrolyzed to dextrin, maltotetroses, maltotrioses, maltose, and glucose in trace amount using amylases. To be suitable commercially, the amylase should be stable at higher temperatures and a wide range of pH values. Moreover, it should also exhibit resistance against a range of chelators, surfactants, and denaturants. It is quite strenuous to isolate an amylase harboring all the required qualities. Therefore, various strategies have been employed to achieve the required attributes. In the present mini‐review, amylase immobilization on conventional matrices as well as on different types of nanomaterials has been discussed with a special emphasis on improvement in the stability and reusability. The stability of the nanomaterials against various physical, chemical, and biological stress is a key factor to be explored commercially. The mini‐review includes mainly magnetite, non‐magnetite, and hybrid (organic–inorganic) nanomaterials. Conclusively, it is anticipated that the advanced strategies would help to overcome the technical barriers in the industrial sectors for better continuous applications of microbial amylases. Abstract : Microbial α‐amylases are extensively used commercially, where more robust and stable α‐amylases are needed for efficient starch hydrolysis under extreme conditions. Immobilization of amylases may improve their stability and catalytic attributes for continuous starch hydrolysis. Magnetite, non‐magnetite, and hybrid (organic–inorganic) nanomaterials may prove to be an efficient supports to achieve the purpose with better immobilization yields.