Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. (30th July 2021)

• Record Type: Journal Article
• Authors: Kostić, Aleksandar Ž.; Milinčić, Danijel D.; Stanisavljević, Nemanja S.; Gašić, Uroš M.; Lević, Steva; Kojić, Milan O.; Lj. Tešić, Živoslav; Nedović, Viktor; Barać, Miroljub B.; Pešić, Mirjana B.
• Is Part Of: Food chemistry. Volume 351(2021)
• Publication Date: 2021-07-30
• Subjects: Monofloral sunflower bee-collected pollen -- Goat milk/pollen powder -- In vitro polyphenol bioaccessibility -- Antioxidant properties; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.129310
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: HPLC analysis showed that TTSG milk/pollen powder is good source of polyphenols. After in vitro digestion of sample, the total polyphenols recovery was 30.71%. TTSG milk/pollen powder exhibited good antioxidant properties. TTSG milk/pollen powder could be a good functional ingredient. Abstract: The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7–76.2%) followed by phenolic acids (14.2–17.4%). Among individual compounds quercetin-3- O -glucoside (155.1–197.2 μg/L) and p -coumaric acid (29.5–30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS + scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS + radicals and to form chelating complexes with Fe 2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.