Baijiu vinasse as a new source of bioactive peptides with antioxidant and anti-inflammatory activity. (1st March 2021)

• Record Type: Journal Article
• Authors: Peng, Lin; Kong, Xiaoyong; Wang, Zongmin; Ai-lati, Aisikaer; Ji, Zhongwei; Mao, Jian
• Is Part Of: Food chemistry. Volume 339(2021)
• Publication Date: 2021-03-01
• Subjects: Baijiu vinasse -- Bioactive peptides -- Antioxidant -- Anti-inflammatory -- Reactive oxygen species; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.128159
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Alkaline extraction assisted ultrasound enhanced vinasse protein yield to 38.72% Peptide fraction VPH-3 (<3 kDa) had high scavenging activity of ROS and RNS radical. VPH-3 peptides inhibited the production of NO and pro-inflammatory cytokines. Sequences of 5 anti-inflammatory peptides were identified by LC-MS/MS analysis. Five identified peptides exhibited anti-inflammatory activity at 0.25 mg/mL. Abstract: During production in Chinese baijiu fermentation process, huge amounts of the by-product vinasse are generated and generally utilized as low-value animal feed. We applied alkaline extraction in combination with ultrasonication to recover vinasse proteins, which were then hydrolyzed by complex protease Corolase PP for 8 h to obtain peptide fractions (VPH-1, -2, -3) displaying high DPPH radical scavenging activity. VPH-3 (<3 kDa) separated by ultrafiltration had EC50 values lower than those of VPH-1 and -2 for reactive oxygen species (ROS) and reactive nitrogen species (RNS) radicals, and significantly inhibited production of NO and pro-inflammatory cytokines in LPS-stimulated RAW264.7 macrophage cells. Active peptides and their amino acid sequences were identified by LC-MS/MS analysis, and five synthesized peptides (particularly KLPDHPKLPK and VDVPVKVPYS) displayed strong anti-inflammatory activity at concentration 0.25 mg/mL. These findings will be useful in future commercial development of baijiu vinasse, including application as a new source of bioactive peptides.