Dietary fiber of Tartary buckwheat bran modified by steam explosion alleviates hyperglycemia and modulates gut microbiota in db/db mice. (July 2022)

• Record Type: Journal Article
• Authors: He, Xiaoqin; Li, Weizhou; Chen, Yuanyuan; Lei, Lin; Li, Fuhua; Zhao, Jichun; Zeng, Kaifang; Ming, Jian
• Is Part Of: Food research international. Volume 157(2022)
• Publication Date: 2022-07
• Subjects: Tartary buckwheat bran -- Soluble dietary fiber -- Steam explosion -- Hyperglycemia -- Insulin resistance -- Gut microbiota -- SCFAs-GPR43 signaling axis -- PI3K/Akt pathway; T2DM Type 2 diabetes mellitus -- PI3K Phosphatidylinositol 3 kinase -- Ins R Insulin receptor -- FoxO1 Forkhead transcription factor O1 -- SCFAs Short-chain fatty acids -- GLP-1 Glucagon like peptide-1 -- GPR43 G protein coupled receptor 43 -- GPR41 G protein coupled receptor 41 -- AMPK Adenosine Monophosphate Activated Protein Kinase -- p-AMPK Phospho-Adenosine Monophosphate Activated Protein Kinase -- PEPCK Phosphoenolpyruvate carboxykinase -- G-6-Pase Glucose-6-phosphatase -- SDF Soluble dietary fiber -- SE Steam explosion -- SE-SDF Steam explosion modified Tartary buckwheat bran SDF -- GHb Glycosylated hemoglobin -- LPS Lipopolysaccharides -- FINS Fasting insulin -- BCA Bicinchoninic acid -- NC Normal control group -- MC Model control group -- PC Positive control group -- SE-SDF 500 SE-SDF intervention at the dose of 500 mg/kg -- SE-SDF 1500 SE-SDF intervention at the dose of 1500 mg/kg -- FBG Fasting blood glucose -- ELISA Enzyme linked immunosorbent assay -- OGTT Oral glucose tolerance test -- AUC Area under the curve -- HOMA-IR Homeostasis index of insulin resistance -- H&E Hematoxylin and eosin -- IOD Integrated optical density -- SEM Standard error of the mean -- ANOVA One-way analysis of variance -- OTUs Operational classification units -- PCA Principal coordinate analysis -- LEfSe Linear discriminant analysis effect size -- F/B The ratio of Firmicutes to Bacteroidetes; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111386
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: SE-SDF of buckwheat bran lowered blood glucose in diabetic db/db mice. SE-SDF of buckwheat bran modulated the gut microbiota in diabetic db/db mice. SE-SDF of buckwheat bran reduced insulin resistance via activation of liver PI3K/Akt/FoxO1 and GPR43/AMPK pathways. SE-SDF of buckwheat bran reduced blood glucose via regulation of SCFAs/GPR43/GLP-1 signal axis. Abstract: Type 2 diabetes is a serious threat to human health. Tartary buckwheat bran dietary fiber has good hypoglycemic activity, with its modification widely studied. However, the hypoglycemic activity of steam explosion modified Tartary buckwheat bran soluble dietary fiber (SE-SDF) has not been reported. This research aimed at investigating the hypoglycemic effect with its underlying mechanism of SE-SDF on type 2 diabetic db/db mice. Results found SE-SDF decreased the levels of fasting blood glucose and glycosylated hemoglobin while improved oral glucose tolerance, insulin resistance, and injuries of liver, pancreas, and colon in diabetic db/db mice. Additionally, SE-SDF up-regulated the protein expression levels of hepatic phosphatidylinositol 3 kinase (PI3K), G protein-coupled receptor43 (GPR43), and phospho-adenosine monophosphate activated protein kinase (p-AMPK), whereas inhibited the protein expression levels of hepatic fork-head transcription factor O1 (FoxO1), phosphoenolpyruvate carboxy kinase (PEPCK) and glucose-6-phosphatase (G-6-Pase). Moreover, SE-SDF increased the production of fecal short chain fatty acids (SCFAs) and the expression of colon GPR43 and the concentration of serum glucagon like peptide-1 (GLP-1), leading to reduced ratio of Firmicutes / Bacteroidetes but increased relative abundance of Parabacteroides, norank_f_ Muribaculaceae, Alloprevotella, Ruminiclostridium _9, unclassified_f _Ruminococcaceae, and Lachnospiraceae _NK4A136_group. These findings suggested that SE-SDF ameliorated type 2 diabetes via activating the liver PI3K/Akt/FoxO1 and GPR43/AMPK signaling pathways and modulating the gut microbiota-SCFAs-GPR43/GLP-1 signaling axis.