Docosahexaenoic acid-rich fish oil alleviates hepatic steatosis in association with regulation of gut microbiome in ob/ob mice. (July 2022)

• Record Type: Journal Article
• Authors: Al-Bulish, Mohammed Said Moose; Cao, Wanxiu; Yang, Ruili; Wang, Yuming; Xue, Changhu; Tang, Qingjuan
• Is Part Of: Food research international. Volume 157(2022)
• Publication Date: 2022-07
• Subjects: Hepatic steatosis -- Docosahexaenoic acid -- Lipid droplet -- Gut microbiome -- Lipid synthesis -- SCFAs; ACAT acyl-CoA: cholesterol acyltranserases -- ANCOM analysis of composition of microbiomes -- CE cholesteryl esters -- DGAT acyl-CoA-dependent diacylglycerol acyltransferase -- DHA docosahexaenoic acid -- EPA Eicosapentaenoic acid -- FO fish oil -- Fsp27 fat-specific protein 27 -- GC gas chromatography -- H&E hematoxylin and eosin -- HDL-C high-density lipoprotein cholesterol -- KEGG Kyoto Encyclopedia of Genes and Genomes -- LD lipid droplets -- LDL-C low-density lipoprotein cholesterol -- NAFLD nonalcoholic fatty liver disease -- NEFA nesterified free fatty acids -- NMDS Non-metric multidimensional scaling -- Plin perilipin -- PUFAs polyunsaturated fatty acids -- SCFAs short chain fatty acids -- SpiecEasi SParse InversE Covariance Estimation for Ecological Association Inference -- SREBP sterol-regulatory element binding protein -- TC total cholesterol -- TG triglyceride -- WGCNA Weighted Gene Correlation Network Analysis -- WT wild type; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111373
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: DHA-rich fish oil (DHA-FO) relieved hepatic steatosis in ob/ob mice. DHA-FO suppressed the biosynthesis of triglyceride and cholesteryl ester. DHA-FO regulated gut microbiome associated with lipid metabolism. Abstract: It remains to study whether docosahexaenoic acid-rich fish oil (DHA-FO) improves hepatic lipid metabolism by leptin-independent mechanisms. We used ob/ob mice as a model to investigate the effects of DHA-FO on hepatic steatosis. DHA-FO inhibited lipid droplets (LD) formation in liver of ob/ob mice. Probably because DHA-FO consumption prevented the accumulation of oleic acid, and suppressed the synthesis of triglycerides and cholesteryl esters. These beneficial effects might be concerned with the promotion of short chain fatty acids (SCFAs) production. Furthermore, DHA-FO could reverse gut bacteria dysbiosis, including increasing the abundance of SCFAs producers (e.g. Akkermansia and unclassified_Muribaculaceae), and suppressing the proliferation of conditional pathogenic bacteria, such as unclassified_Lachnospiraceae. DHA-FO also promoted colonic microbial function ("Glycerolipid metabolism") associated with lipid metabolism. As a potential ingredient for functional food, DHA-FO reduced LD accumulation, which might be associated with modulation of obesity-linked gut microbiome in ob/ob mice.