Ergosterol isolated from cloud ear mushroom (Auricularia polytricha) attenuates bisphenol A-induced BV2 microglial cell inflammation. (July 2022)

• Record Type: Journal Article
• Authors: Sillapachaiyaporn, Chanin; Chuchawankul, Siriporn; Nilkhet, Sunita; Moungkote, Nuntanat; Sarachana, Tewarit; Ung, Alison T.; Baek, Seung Joon; Tencomnao, Tewin
• Is Part Of: Food research international. Volume 157(2022)
• Publication Date: 2022-07
• Subjects: Bisphenol A -- Auricularia polytricha -- Ergosterol -- Neuroinflammation; BPA Bisphenol A -- AP Auricularia polytricha -- APH Hexane extract of AP -- APE Ethanol extract of AP -- APW Water extract of AP -- AD Alzheimer's disease -- RT-qPCR reverse transcription-quantitative real-time PCR -- ELISA enzyme-linked immunosorbent assay -- NO Nitric oxide -- TNF-α Tumor necrosis factor alpha -- IL1-β interleukin 1-β; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111433
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Mushroom extract and its bioactive component ergosterol attenuated BPA-induced neuroinflammation in BV2 microglial cells. Extracts and ergosterol from Auricularia polytricha demonstrated anti-neuroinflammatory activities by suppressing the NF-kB activity and increasing SOD expression. Extracts from Auricularia polytricha treated on BPA-induced BV2 cells attenuated HT-22 hippocampal cell damage. Abstract: Bisphenol A (BPA) has been reported to have neurotoxic properties that may increase the risk of neurodegenerative diseases by inducing neuroinflammation. Auricularia polytricha (AP) is an edible mushroom with several medicinal properties. Herein, the anti-neuroinflammatory effects of AP extracts against BPA-induced inflammation of BV2 microglial cells were investigated. Hexane (APH) and ethanol (APE) extracts of AP inhibited BPA-induced neuroinflammation in BV2 microglia by reducing microglial activation and the expression of pro-inflammatory cytokines. These anti-inflammatory effects were regulated by the NF-κB signaling pathway. In addition, APH and APE exhibited antioxidative effects by increasing the activity of the SOD-1 enzyme and restoring the accumulation of reactive oxygen species (ROS) in BPA-induced BV2 cells. Moreover, the conditioned medium prepared using BPA-induced BV2 cells demonstrated that the presence of APH or APE could attenuate ROS production in HT-22 cells. Further, ergosterol was isolated from APE and also showed anti-inflammatory and antioxidative activities. In conclusion, AP extracts and ergosterol attenuated neuroinflammation against BPA induction in BV2 microglial cells through the NF-κB signaling pathway.