Lactobacillus plantarum ZY08 relieves chronic alcohol-induced hepatic steatosis and liver injury in mice via restoring intestinal flora homeostasis. (July 2022)

• Record Type: Journal Article
• Authors: Ding, Qinchao; Cao, Feiwei; Lai, Shanglei; Zhuge, Hui; Chang, Kaixin; Valencak, Teresa G.; Liu, Jianxin; Li, Songtao; Ren, Daxi
• Is Part Of: Food research international. Volume 157(2022)
• Publication Date: 2022-07
• Subjects: Lactobacillus plantarum ZY08 -- Alcoholic liver disease -- Intestinal flora homeostasis -- Intestinal barrier function -- Hepatic steatosis -- Liver injury; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111259
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Lactobacillus plantarum ZY08 is a kind of probiotic isolated from local baby feces. L. plantarum ZY08 attenuates hepatic steatosis and liver injury in alcohol-fed mice. L. plantarum ZY08 treatment improves alcohol-destroyed intestinal barrier and gut microbiota dysbiosis, and elevates intestinal short-chain fatty acid (SCFA) content. ABX gavage test proves the regulative role ZY08 on intestinal flora mechanistically contributed to its protective role against ALD. Abstract: This present study was designed to test the protective role of two Lactobacillus plantarum strains, E680 and ZY08, against alcoholic liver disease (ALD) in C57BL/6 mice. The ALD mouse model was established by exposing the mice to a Lieber-DeCarli ethanol liquid diet. The two probiotic strains (10 9 cfu/day) were administered by oral gavage, respectively. Our data showed that L. plantarum ZY08, but not E680, intervention significantly mitigated alcohol-related hepatic steatosis, liver injury, intestinal barrier, and it alleviated plasma endotoxin (LPS) levels, and affected hepatic genes relating to lipid metabolism. Furthermore, Lactobacillus plantarum ZY08 effectively restored intestinal flora homeostasis via recovering flora abundance, including Blautia, Oscillibacter, Lachnoclostridium and Intestimonas, and consequently elevated intestinal short-chain fatty acid (SCFA) content. More importantly, removing intestinal microorganisms through ABX gavage markedly abolished the beneficial aspects of Lactobacillus plantarum ZY08, indicating that the regulative role of Lactobacillus plantarum ZY08 contributed to its protective role against ALD. Overall, Lactobacillus plantarum ZY08 is a potential candidate for mitigating alcohol-induced hepatic steatosis and liver injury.