Buglossoides spp. seeds, a land source of health-promoting n-3 PUFA and phenolic compounds. (July 2022)

• Record Type: Journal Article
• Authors: Chelh, Tarik Chileh; Lyashenko, Svetlana; Lahlou, Abdallah; Belarbi, El-Hassan; Rincón-Cervera, Miguel Ángel; Rodríguez-García, Ignacio; Urrestarazu-Gavilán, Miguel; López-Ruiz, Rosalía; Guil-Guerrero, José Luis
• Is Part Of: Food research international. Volume 157(2022)
• Publication Date: 2022-07
• Subjects: 1H NMR proton Nuclear Magnetic Resonance -- ANOVA one-way analysis of variance -- ALA α-linolenic acid -- Csa hot-summer Mediterranean climate -- Csb warm-summer Mediterranean climate -- DHA docosahexaenoic acid -- DMSO dimethylsulfoxide -- DPA docosapentaenoic acid -- EA erucic acid -- FA fatty acid -- ELISA enzyme-linked immunosorbent assay -- EPA eicosapentaenoic acid -- FAME FA methyl ester -- GC gas chromatography -- GLA γ-linolenic acid -- GOA gondoic acid -- LA linoleic acid -- LC-MS LC-mass spectrometry -- LOD limit of detection -- LOQ limit of quantification -- MUFA monounsaturated FA -- MTT 3-(4:5-dimethylthiazol-2-yl)-2:5-diphenyltetrazolium bromide -- OA oleic acid -- PA palmitic acid -- PCA Principal-components analysis -- POA palmitoleic acid -- PUFA polyunsaturated FA -- SA stearic acid -- SDA stearidonic acid -- SFA saturated FA -- SI selectivity index -- UFA unsaturated FA -- VA vaccenic acid -- VLCPUFA very long-chain PUFA; Buglossoides spp. -- γ-linolenic acid -- Stearidonic acid -- Phenolic compounds -- HT-29 cells; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111421
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Seeds of several Buglossoides taxa were surveyed for fatty acids (FA) and phenolics. Stearidonic acid reached high amounts in wild B. arvensis samples. Rosmarinic and lithospermic acids were the predominant phenolics. Unsaturated FA allow grouping Buglossoides taxa as botanical criteria do. The phenolics of B. arvensis were active against HT-29 colorectal cancer cells. Abstract: Ahiflower oil© is extracted from the seeds of Buglossoides arvensis, which contains high amounts of stearidonic acid (SDA, 18:4 n -3), while its phenolic composition still is unreported. Moreover, several Buglossoides taxa remain unstudied and could become natural sources of SDA. In this work, seeds of several Buglossoides taxa and Ahiflower oil© were screened for fatty acids, phenolic compounds, and in vitro antiproliferative activities against colorectal cancer cells. Four flavonoids and 16 phenolic acids were identified and quantified. Among Buglossoides taxa, the highest amounts of phenolic compounds were found in samples collected in Spain, under a warm Mediterranean climate. Rosmarinic and lithospermic acids were the main phenols found in Buglossoides seeds. The MTT assay showed dose- and time-dependent inhibitory effects of B. arvensis extracts on HT-29 cancer cells, with a GI50 value of ∼280 μg/mL after 72 h of cell exposure to seed extracts. The latter showed lower antiproliferative activity than that of pure phenolics due to the simultaneous presence of other compounds in the extracts, as evidenced by 1 H NMR. This work constitutes the first approach to evaluate the seeds of several Buglossoides taxa as functional oils-providers to use them as functional foods.