Nanocomplexes composed of chitosan derivatives and β-Lactoglobulin as a carrier for anthocyanins: Preparation, stability and bioavailability in vitro. (February 2019)

• Record Type: Journal Article
• Authors: Ge, Jiao; Yue, Xueyang; Wang, Shuo; Chi, Jinpeng; Liang, Jin; Sun, Yue; Gao, Xueling; Yue, Pengxiang
• Is Part Of: Food research international. Volume 116(2019)
• Page Start: 336
• Page End: 345
• Publication Date: 2019-02
• Subjects: Anthocyanins -- Chitosan -- β-Lactoglobulin -- Release -- Stability -- Bioavailability; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2018.08.045
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: To improve sustained-release property, stability and bioavailability of anthocyanins (ACNs) in vitro, we fabricated the nanocomplexes with chitosan hydrochloride (CHC), carboxymethyl chitosan (CMC) and β-Lactoglobulin (β-Lg). Response surface methodology (RSM) combined with desirability function was employed to optimize ACNs-loaded chitosan/β-Lg (CHC/CMC expressed with chitosan) nanocomplexes with maximum anthocyanins retention rate, preferred particle size and high encapsulation efficiency. The result suggested that the optimized conditions were 5.16 mg/mL of β-Lg, 1.45 mg/mL of CMC and 6.09 of pH CMC solution. Based on optimized conditions, anthocyanins retention rate, particle size and encapsulation efficiency of ACNs-loaded chitosan/β-Lg nanocomplexes were 68.9%, 91.71 nm and 69.33%, respectively. ACNs-loaded chitosan/β-Lg nanocomplexes was more stable in simulated gastric fluid (SGF, pH 1.2) and simulated intestinal fluid (SIF, pH 6.8) by showing less ACNs release (%) than that ACNs solution and ACNs-loaded CHC/CMC nanocomplexes. Further, stability and bioavailability of ACNs in simulated gastrointestinal (GI) tract were significantly improved by nanocomplexes encapsulation. Compared with ACNs-loaded CHC/CMC nanocomplexes, ACNs-loaded chitosan/β-Lg nanocomplexes displayed better sustained ACNs release, stability and bioavailability. Graphical abstract: Unlabelled Image Highlights: The complex of CHC, CMC and β-Lg was first used as wall materials of ACNs-loaded. RSM combined with desirability function was employed to optimize nanocomplexes. Nanocomplexes displayed better sustained-release property in SGF and SIF. Nanocomplexes improved the stability and bioavailability of ACNs in GI conditions.