Α-Linolenic acid rich-chia seed modulates visceral adipose tissue collagen deposition, lipolytic enzymes expression, insulin signaling and GLUT-4 levels in a diet-induced adiposity rodent model. (June 2022)

• Record Type: Journal Article
• Authors: Aiassa, Victoria; del Rosario Ferreira, María; Villafañe, Noelia; Eugenia D'Alessandro, María
• Is Part Of: Food research international. Volume 156(2022)
• Publication Date: 2022-06
• Subjects: ALA α-linolenic acid -- ATGL adipocyte triglyceride lipase -- DHA docosahexaenoic acid -- EPA eicosapentaenoic acid -- eWAT epididymal white adipose tissue -- FFA free fatty acids -- GIR glucose infusion rate -- GLUT-4 glucose transporter-4 -- H&E hematoxylin & eosin -- HSL hormone-sensitive lipase -- IR insulin resistance -- LA linoleic acid -- n-3 PUFA n-3 polyunsaturated fatty acids -- RD reference diet -- rWAT retroperitoneal white adipose tissue -- SRD sucrose-rich diet -- SRD+CHIA sucrose-rich diet plus chia seed -- TG triglyceride -- VAI visceral adiposity index -- WAT white adipose tissue; Chia seed -- Insulin-resistant rats -- Adipose tissue remodeling -- Lipolysis -- Insulin signaling -- Glucose transport; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111164
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: In SRD-fed rats: Chia seed reduced adipocytes size and collagen deposition in eWAT and rWAT. Chia seed modulated eWAT and rWAT lipolytic enzymes. Chia seed increased pAKT /total AKT ratio in eWAT and rWAT under insulin stimulus. Chia seed modulated fat pads GLUT-4 levels. Abstract: Given obesity and its associated metabolic disorders have reached epidemic proportions, the study of therapeutic strategies targeting white adipose tissue (WAT) are of main research interest. We previously shown that α-linolenic acid–rich chia seed was able to ameliorate a wide range of metabolic disorders including body fat accretion in sucrose-rich diet (SRD)-fed rats, an experimental model of visceral adiposity and insulin resistance. However, the mechanisms involved are not fully clarified. The aim of this study was to evaluate the effect of chia seed administration upon WAT remodeling and key enzymes that controls lipolysis, insulin signaling (tAKT, pAKT), and GLUT-4 levels in different visceral fat pad depots (epididymal –eWAT- and retroperitoneal –rWAT- adipose tissues) of SRD-fed rats. Results showed that chia seed reduces adipocytes hypertrophy, the increased lipid content and collagen deposition in both WAT. These changes were accompanied by a significant reduction of HSL and ATGL protein levels in eWAT and HSL protein levels in rWAT. Moreover, chia seed restored the altered expression pattern of the pAKT observed in SRD-fed rats, and modulated GLUT-4 levels. Chia seed could be a dietary intervention of great relevance with potential beneficial effects in the management of body fat excess and WAT function.