Combined metabolome and transcriptome analysis reveal the mechanism of selenate influence on the growth and quality of cabbage (Brassica oleracea var. capitata L.). (June 2022)

• Record Type: Journal Article
• Authors: Yang, Xiaoyan; Liao, Xiaoli; Yu, Li; Rao, Shen; Chen, Qiangwen; Zhu, Zhenzhou; Cong, Xin; Zhang, Weiwei; Ye, Jiabao; Cheng, Shuiyuan; Xu, Feng
• Is Part Of: Food research international. Volume 156(2022)
• Publication Date: 2022-06
• Subjects: Brassica oleracea -- Selenate -- Glucosinolate -- Flavonoids -- Phenolic acid -- Metabolome -- Transcriptome; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111135
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Low concentration of selenate (0.1 mmol/L) promoted growth and nutritional quality of cabbage. SeCys2 and SeMet were the representative organic Se species in cabbage. Selenate treatment induced selenocompound and correlated genes up-regulated. Glucosinolates, flavonoids and phenolic acids directly participated in cabbage response to selenate. Comparative transcriptome and metabolomics analysis revealed the crucial genes involve in cabbage response to selenate. Abstract: Selenium is an essential trace element for human and animal health, and an appropriate amount of Se can promote the growth and development of plants. Cabbage is a popular cruciferous vegetable with a good ability to accumulate Se, and Se-enriched cabbage can be used as an important Se source for humans. However, the effects of Se-enriched cultivation and the Se accumulation mechanism in cabbage are still unclear. In this study, the effects of different concentrations (0, 0.1, 0.2, 0.4, 0.8, and 1.6 mmol/L) of selenate on cabbage growth and quality were explored. A low concentration of selenate (0.1 mmol/L) promoted growth and nutritional quality. The contents of total Se, S, selenocystine, and selenomethionine significantly increased following selenate application. Important secondary metabolites, namely glucosinolates, phenolic acids, and flavonoids, participate in the response to selenate in cabbage. Comparative transcriptome and metabolomics analysis revealed that SULTR2.2, SULTR3.1, APS, APK2, HMT, MMT, and NTR2 played important roles in Se absorption and conversion. Additionally, the SUR1, UGT74B1, and ST5b genes and cytochrome P450 family genes CYP83A1, CYP79A2, and CYP79F1 may be the crucial genes in the glucosinolates biosynthesis and regulation pathway. The PAL, 4CL, CAD, CHS3, FLS, and CYP73A5 genes were involved in flavonoid and phenolic acid accumulation under selenate treatment. These results reveal the internal relationships in the regulatory network of Se metabolism and secondary metabolite biosynthesis in cabbage and help further the understanding of the physiological and molecular mechanism of how Se biofortification affects cabbage quality, thereby providing genetic resources and a technical basis for the breeding and cultivation of Se-enriched cabbage with excellent nutritional quality.