Encapsulation of selenium-containing peptides in xanthan gum-lysozyme nanoparticles as a powerful gastrointestinal delivery system. (June 2022)

• Record Type: Journal Article
• Authors: Zhu, Yiqing; Sun, Xinyang; Luo, Xieqi; Ding, Jian; Fan, Fengjiao; Li, Peng; Shen, Xinchun; Fang, Yong
• Is Part Of: Food research international. Volume 156(2022)
• Publication Date: 2022-06
• Subjects: Nanoparticles -- Encapsulation -- Se-containing peptides -- Gastrointestinal delivery; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111351
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Nanoparticles (NPs) effectively encapsulated Se-containing peptides (STP and SHP). NPs-STP and NPs-SHP exhibited good colloidal stability and antioxidant activity. The release of Se-containing peptides was controlled during in vitro digestion. NPs-STP and NPs-SHP significantly enhanced cellular uptake of Se-containing peptides. Abstract: In this study, nanoparticles (NPs) prepared with xanthan gum and lysozyme were established as a powerful delivery system for two Se-containing peptides: TSeMMM (STP) and SeMDPGQQ (SHP). NPs-STP and NPs-SHP had relatively small particle sizes (145 nm and 148 nm) and negative zeta potentials (−47 mV and −49 mV). The encapsulation efficiency of NPs-STP and NPs-SHP was determined to be 34.35% and 41.35%, respectively. The stability and antioxidant activity of Se-containing peptides were greatly enhanced due to encapsulation. NPs-STP and NPs-SHP exhibited controlled release of Se-containing peptides under in vitro gastrointestinal conditions. NPs-STP and NPs-SHP showed low toxicity and entered Caco-2 cells through clathrin-mediated endocytosis, contributing to a significant increase in the apparent permeability coefficient of STP (2.19 × 10 −6 cm/s) and SHP (2.21 × 10 −6 cm/s). Thus, NPs-STP and NPs-SHP are considered promising delivery systems for Se-containing peptides and have good potential applications in the food and pharmaceutical industries.