A simple isothermal nucleic acid amplification method for the effective on-site identification for adulteration of pork source in mutton. (April 2019)

• Record Type: Journal Article
• Authors: Liu, Rui; Wang, Xiudan; Wang, Xuejiao; Shi, Yanjing; Shi, Chao; Wang, Wei; Ma, Cuiping
• Is Part Of: Food control. Volume 98(2019)
• Page Start: 297
• Page End: 302
• Publication Date: 2019-04
• Subjects: Isothermal nucleic acid amplification -- SEA -- Meat authentication -- Pork -- On-site detection; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2018.11.040
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: As meat adulteration issue is becoming increasingly prominent worldwide, it is crucial to realize on-site detection with simple equipment. Conventional nucleic acid amplification methods are reliable but the requirement of complex equipment, skilled technicians and long operation time limit their on-site use. Here, a simple denaturation bubble-mediated strand exchange amplification method (SEA) requiring only a pair of primers and one polymerase was first reported for identifying adulteration of pork source by targeting the specie-specific mitochondrial DNA sequence. The SEA method displayed good specificity for pork and could detect as low as 30 pg/μL pork DNA. In binary mixtures, the SEA method could detect 1% pork meat total DNA by both colorimetric and fluorescence determination, fulfilling the requirement of artificial meat adulteration. Excitedly, the whole detection process could be finished within 1 h by coupling with fast tissue DNA extraction method, only requiring a simple heating block. Therefore, with simplicity and rapidity, SEA method will be suitable for on-site meat species identification.