UPLC-MS metabolome based classification of Lupinus and Lens seeds: A prospect for phyto-equivalency of its different accessions. (January 2019)

• Record Type: Journal Article
• Authors: Farag, Mohamed A.; Khattab, Amira R.; Maamoun, Amal A.; Kropf, Matthias; Heiss, Andreas G.
• Is Part Of: Food research international. Volume 115(2019)
• Page Start: 379
• Page End: 392
• Publication Date: 2019-01
• Subjects: Metabolites profiling -- Lens -- Lupinus -- PCA -- OPLS-DA -- Functional foods -- Multivariate data analysis; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2018.11.003
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Fabaceae is well-known for its seed nutritious and bioactive composition as exemplified by Lupinus and Lens . Developing efficient analytical approaches for profiling their bioactive matrix is a prerequisite to provide proof for their health benefits or nutritive traits. Eight Lupinus and Lens seed accessions were subjected to liquid chromatography-mass spectrometry (UPLC-MS)-based metabolomic study, which identified 66 metabolites, viz. flavonoids, alkaloids, saponins, phenolics, fatty acids and sphingolipids. Chemometric tools were explored to assess heterogeneity across the two genera leading to elucidation of the species-most enriched and differential metabolites. The two dark-colored lentil cultivars are identified as the richest source of functional foods with presumed therapeutic benefits; however, Lupinus hispanicus was proved to be the most nutritive accession. To our knowledge, this study provides the first UPLC-MS-based comparative metabolite profiling of Lupinus and Lens seeds. This platform was also able to discern metabolites diversity at the intraspecific level among Lupinus species and Lens cultivars. Graphical abstract: Unlabelled Image Highlights: UPLC-MS-based comparative metabolite profiling of 8 Lupinus and Lens seeds Chemometric tools assessed heterogeneity across the two genera. Metabolomics was able to discern metabolites diversity at the infra-specific level. Two dark-colored lentil cultivars presented the richest source of natural products.