Ultrasound assisted dispersive liquid-liquid microextraction for fast and accurate analysis of chloramphenicol in honey. (January 2019)

• Record Type: Journal Article
• Authors: Campone, Luca; Celano, Rita; Piccinelli, Anna Lisa; Pagano, Imma; Cicero, Nicola; Sanzo, Rosa Di; Carabetta, Sonia; Russo, Mariateresa; Rastrelli, Luca
• Is Part Of: Food research international. Volume 115(2019)
• Page Start: 572
• Page End: 579
• Publication Date: 2019-01
• Subjects: Honey -- Chloramphenicol quantitative analysis -- UA-DLLME -- Validation Reg. 2002/657 EC -- Experimental design optimisation -- UHPLC MS/MS; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2018.09.006
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: Honey is a food produced from honey bee widely used for the sweetening power and for its biological properties. In order to prevent the infection of the hive, different xenobiotics (antibiotics, pesticide) were frequently employed. One of these substances is the chloramphenicol, that given its chemical stability could often found in food. Chloramphenicol have several side effects in humans after their ingestion and for this reason their intake must be avoid. The aim of this study, was developed an ultrasound-assisted dispersive liquid-liquid microextraction method coupled with UHPLC MS/MS determination, for fast and accurate analysis of chloramphenicol in honey. The parameters affecting on extraction efficiency were carefully optimized using an experimental design in order to maximized the recovery reducing matrix effects. After the optimization the method was validated and successfully applied to 66 honey samples. Graphical abstract: Unlabelled Image Highlights: Fast and easy analysis of chloramphenicol Validation method for the analysis of chloramphenicol in honey Optimization of extraction parameters by response surface design Innovative extraction technique for determination of chloramphenicol in honey.