Effects of UV/H2O2 degradation and step gradient ethanol precipitation on Sargassum fusiforme polysaccharides: Physicochemical characterization and protective effects against intestinal epithelial injury. (May 2022)

• Record Type: Journal Article
• Authors: Yao, Wanzi; Liu, Mengyuan; Chen, Xiaoyong; You, Lijun; Ma, Yongxuan; Hileuskaya, Kseniya
• Is Part Of: Food research international. Volume 155(2022)
• Publication Date: 2022-05
• Subjects: Sargassum fusiforme -- Polysaccharides -- UV/H2O2 -- IEC-6 cells -- Intestinal barrier function -- Intestinal inflammation; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.111093
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: S. fusiforme polysaccharides (SFP) were treated and produced degraded SFP (DSFP). The treatment includes UV/H2 O2 degradation and step gradient ethanol precipitation. DSFP increased cell migration during intestinal epithelial wound healing. DSFP attenuated lipopolysaccharides-induced intestinal inflammation. The treatment improved the protective effects of SFP against intestinal injury. Abstract: In this study, the degraded purified fraction from Sargassum fusiforme polysaccharides (SFP), named DSFP, was produced by the treatment of ultraviolet/hydrogen peroxide (UV/H2 O2 ) degradation and step gradient ethanol precipitation. Results showed that the treatment significantly reduced the molecular weight of polysaccharides, from 282.83 kDa to 18.54 kDa, and influenced their surface morphology and roughness. SFP and DSFP were typical sulfated polysaccharides, mainly composed of fucose, galacturonic acid, glucuronic acid, galactose, and mannose. Both SFP and DSFP increased cell migration during intestinal epithelial wound healing and stimulated the cell cycle progression by promoting the transition from G0/G1 to S phase in the rat intestine epithelium cells (IEC-6). But DSFP had a stronger positive effect on wound healing and cell migration than SFP. It reinforced the intestinal barrier function and attenuated lipopolysaccharides-induced intestinal inflammation. DSFP significantly downregulated the expression of Toll-like receptor 4, tumor necrosis factor-α, interleukin-6, interleukin-1β, and inducible nitric oxide synthase by 53.14%, 92.41%, 66.01%, 68.24%, and 78.09%, respectively, and upregulated that of interleukin-10 by 2.48 folds when compared to the model. Therefore, the treatment (UV/H2 O2 degradation and step gradient ethanol precipitation) could effectively improve the protective effects against intestinal epithelial injury.