Gold core @ platinum shell nanozyme-mediated magnetic relaxation switching DNA sensor for the detection of Listeria monocytogenes in chicken samples. (July 2022)

• Record Type: Journal Article
• Authors: Wu, Zijing; Huang, Chenxi; Dong, Yongzhen; Zhao, Binjie; Chen, Yiping
• Is Part Of: Food control. Volume 137(2022)
• Publication Date: 2022-07
• Subjects: Listeria monocytogenes -- Au@Pt nanozymes -- Magnetic relaxation switching -- Mn (VII)/Mn (II) conversion -- Hydrogen peroxide -- DNA hybridization; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2022.108916
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Herein, an Au@Pt nanozyme-mediated magnetic relaxation switching (MRS) DNA biosensor has been developed for the rapid detection of Listeria monocytogenes . Au@Pt nanoparticle was with enhanced stability and high peroxidase activity for decomposing the hydrogen peroxide. After DNA hybridization and magnetic separation, the conjugates of MNP180 -probe1 - L. monocytogenes DNA-probe2 -Au@Pt could adjust the hydrogen peroxide-mediated Mn(VII)/Mn(II) conversion, and thus leading to the change of the transverse relaxation time. The amount of Au@Pt was directly proportional to the concentration of L. monocytogenes . The Au@Pt nanozyme and the hydrogen peroxide-mediated Mn(VII)/Mn(II) signal conversion system contributed to the sensitivity and stability of this sensor. This sensor enabled the detection of L. monocytogenes with a LOD of 30 CFU/mL, which was 33-folds enhancement compared with that of enzyme-linked immunosorbent assay, providing a promising platform to trace the foodborne pathogen in the complex food matrix. Highlights: An Au@Pt mediated MRS DNA sensor for the rapid detection of L. monocytogenes. This sensor integrates Au@Pt nanozyme with H2 O2 -mediated signal readout. Au@Pt nanozyme was synthesized to replace horseradish peroxidase. H2 O2 -mediated Mn(VII)/Mn(II) signal readout is highly stable and sensitive.