Gold core @ platinum shell nanozyme-mediated magnetic relaxation switching DNA sensor for the detection of Listeria monocytogenes in chicken samples. (July 2022) - Wu, Zijing
Abstract: Herein, an Au@Pt nanozyme-mediated magnetic relaxation switching (MRS) DNA biosensor has been developed for the rapid detection of Listeria monocytogenes . Au@Pt nanoparticle was with enhanced stability and high peroxidase activity for decomposing the hydrogen peroxide. After DNA hybridization and magnetic separation, the conjugates of MNP180 -probe1 - L. monocytogenes DNA-probe2 -Au@Pt could adjust the hydrogen peroxide-mediated Mn(VII)/Mn(II) conversion, and thus leading to the change of the transverse relaxation time. The amount of Au@Pt was directly proportional to the concentration of L. monocytogenes . The Au@Pt nanozyme and the hydrogen peroxide-mediated Mn(VII)/Mn(II) signal conversion system contributed to the sensitivity and stability of this sensor. This sensor enabled the detection of L. monocytogenes with a LOD of 30 CFU/mL, which was 33-folds enhancement compared with that of enzyme-linked immunosorbent assay, providing a promising platform to trace the foodborne pathogen in the complex food matrix. Highlights: An Au@Pt mediated MRS DNA sensor for the rapid detection of L. monocytogenes. This sensor integrates Au@Pt nanozyme with H2 O2 -mediated signal readout. Au@Pt nanozyme was synthesized to replace horseradish peroxidase. H2 O2 -mediated Mn(VII)/Mn(II) signal readout is highly stable and sensitive.