Effects of acylated and nonacylated anthocyanins extracts on gut metabolites and microbiota in diabetic Zucker rats: A metabolomic and metagenomic study. (March 2022)

• Record Type: Journal Article
• Authors: Chen, Kang; Wei, Xuetao; Kortesniemi, Maaria; Pariyani, Raghunath; Zhang, Yumei; Yang, Baoru
• Is Part Of: Food research international. Volume 153(2022)
• Publication Date: 2022-03
• Subjects: Acylated anthocyanins -- Nonacylated anthocyanins -- Non-targeted 1H NMR metabolomics -- Gut microbiota -- Type 2 diabetes; AAPP acylated anthocyanins extracted from purple potato -- DMA dimethylamine -- MA methylamine -- NAAB nonacylated anthocyanins extracted from bilberry -- ND normal diet -- SCFA short-chain fatty acids -- T2D type 2 diabetes -- ZDF Zucker diabetic fatty -- TMA trimethylamine; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2022.110978
• Languages: English
• ISSNs: 0963-9969

Abstract

Graphical abstract: Highlights: Dysbiosis of gut microbiota and metabolites in diabetic rat were observed. Anthocyanins were extracted from purple potato and bilberry. Acylated and nonacylated anthocyanins increased cecal sugars. Acylated anthocyanins modulated gut oxidative phosphorylation and purine metabolism. 50 mg/kg acylated anthocyanins increased cecal short-chain fatty acids. Abstract: Anthocyanins have been shown to have prebiotic properties. This study investigated the impact of nonacylated anthocyanins and acylated anthocyanins on fecal and cecal metabolites and colonic gut microbiota in diabetic state using 1 H nuclear magnetic resonance (NMR) metabolomics and metagenomic sequencing. Zucker diabetic fatty rats fed with high-fat diet were gavaged with nonacylated anthocyanins extracted from bilberries (NAAB) or acylated anthocyanins extracted from purple potatoes (AAPP) at daily doses of 25 and 50 mg/kg body weight for 8 weeks. Lean Zucker rats fed with normal diet (ND) and high-fat diet (Con) were used as healthy controls groups. Binned NMR spectra and sequenced gene abundance were used for data analysis. Dysbiosis of colonic microbiota and gut metabolites in the diabetic rats were observed compared to the lean Zucker rats. Both anthocyanin extracts increased cecal sugar levels and the abundance of Peptostreptococcaceae sp. and decreased the abundance of Parabacteroides spp. in colon. In addition to the increased fecal short-chain fatty acids, AAPP decreased colonic Ruminococcus torques and Lachnospiraceae bacterium 4_1_37FAA abundances and increased oxidative phosphorylation. The anthocyanin extracts modulated the gut metabolism and microbiota in diabetes, with AAPP showing more regulatory and beneficial effects on diabetes.